Kubota H, Reid L M
Department of Physiology, Program in Molecular Biology and Biotechnology, University of North Carolina School of Medicine 27599-7038, USA.
Hum Cell. 1997 Mar;10(1):51-62.
Stem cell-fed maturational lineages have long known to exist in rapidly proliferating tissues such as bone marrow, gut and epidermis. Recent studies support the hypothesis that stem cell-fed maturational lineages occur also in quiescent tissues. In this review is presented evidence for this hypothesis using liver as one of the model systems representative of quiescent tissues. In addition, studies are summarized indicating that control of growth and tissue-specific gene expression is dependent on maturational lineage mechanisms operating dynamically and sometimes synergistically in combination with gradients of regulatory signals that include hormones, growth factors and extracellular matrix components. Tissue engineering for optimal maintenance of cells and tissues ex vivo and for the development of bioartificial organs will depend on use of cells at specific maturational lineage stages, seeding them onto substrata of specific mixtures of extracellular matrix components, and culturing them in hormonally and nutritionally defined media.
长期以来，人们已知干细胞滋养的成熟谱系存在于骨髓、肠道和表皮等快速增殖的组织中。最近的研究支持这样一种假说，即干细胞滋养的成熟谱系也存在于静止组织中。在本综述中，以肝脏作为静止组织的代表性模型系统之一，给出了支持这一假说的证据。此外，研究总结表明，生长和组织特异性基因表达的调控依赖于成熟谱系机制，这些机制有时与包括激素、生长因子和细胞外基质成分在内的调节信号梯度动态协同作用。为了在体外最佳地维持细胞和组织以及开发生物人工器官，组织工程将依赖于使用处于特定成熟谱系阶段的细胞，将它们接种到细胞外基质成分特定混合物的基质上，并在激素和营养成分明确的培养基中培养。
