Awasthi V, Gambhir S, Sewatkar A B
Department of Nuclear Medicine, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow (U.P.), India.
Nucl Med Biol. 1994 Feb;21(2):251-4. doi: 10.1016/0969-8051(94)90016-7.
Porcine insulin was labeled with 99mTc by direct tin reduction. More than 95% labeling efficiency was obtained on paper chromatography in saline and methyl ethyl ketone. The stability of the labeled compound was confirmed by paper chromatography at 3 h post-labeling and by human serum albumin (HSA) challenge. PAGE pattern indicated no change in the electrophoretic behavior and the molecular size of insulin after the labeling procedure. Biodistribution in rats shows that kidney took up the maximum amount of 99mTc-insulin; maxima being maintained throughout 24 h post-injection. Liver and intestine were the other organs with significant uptake; the rest localizing little or negligible radioactivity. Most of the radioactivity was excreted via the renal pathway into urine. Scintiimages conformed to the biodistribution data. The results of this study present the potential of 99mTc-labeling of insulin by a simple method.
通过直接锡还原法用99mTc标记猪胰岛素。在生理盐水和甲乙酮的纸层析中获得了超过95%的标记效率。标记后3小时通过纸层析和人血清白蛋白（HSA）激发确认了标记化合物的稳定性。聚丙烯酰胺凝胶电泳（PAGE）图谱表明标记过程后胰岛素的电泳行为和分子大小没有变化。大鼠体内生物分布显示，肾脏摄取的99mTc-胰岛素量最大；在注射后24小时内一直保持最大值。肝脏和肠道是其他有明显摄取的器官；其余器官的放射性定位很少或可忽略不计。大部分放射性通过肾脏途径排泄到尿液中。闪烁图像与生物分布数据一致。本研究结果展示了通过一种简单方法用99mTc标记胰岛素的潜力。
