Ulmer J B, Deck R R, DeWitt C M, Fu T M, Donnelly J J, Caulfield M J, Liu M A
Department of Virus and Cell Biology, Merck Research Laboratories, West Point, PA 19486, USA.
Vaccine. 1997 Jun;15(8):839-41. doi: 10.1016/s0264-410x(96)00256-3.
Intramuscular injection of plasmid DNA expression vectors results in transfection of myocytes in situ. To determine whether expression of antigen by myocytes is sufficient to induce protective cell-mediated immunity, stably transfected myoblasts expressing influenza nucleoprotein (NP) were transplanted into mice. These animals produced high-titer anti-NP antibodies and MHC class I-restricted cytotoxic T lymphocytes, and were protected from a cross-strain lethal challenge with influenza A virus. Therefore, antigen expression by muscle cells in vivo is sufficient to confer protective cell-mediated immunity.
肌内注射质粒DNA表达载体可导致原位肌细胞转染。为了确定肌细胞对抗原的表达是否足以诱导保护性细胞介导的免疫，将稳定转染表达流感核蛋白（NP）的成肌细胞移植到小鼠体内。这些动物产生了高滴度的抗NP抗体和MHC I类限制性细胞毒性T淋巴细胞，并受到甲型流感病毒跨株致死性攻击的保护。因此，体内肌肉细胞的抗原表达足以赋予保护性细胞介导的免疫。
