Maloy K J, Erdmann I, Basch V, Sierro S, Kramps T A, Zinkernagel R M, Oehen S, Kündig T M
Department of Dermatology, and Institute of Experimental Immunology, Universitätsspital Zürich, Schmelzbergstrasse 12, CH-8091 Zurich, Switzerland.
Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3299-303. doi: 10.1073/pnas.051630798. Epub 2001 Feb 27.
Although DNA vaccines have been shown to elicit potent immune responses in animal models, initial clinical trials in humans have been disappointing, highlighting a need to optimize their immunogenicity. Naked DNA vaccines are usually administered either i.m. or intradermally. The current study shows that immunization with naked DNA by direct injection into a peripheral lymph node enhances immunogenicity by 100- to 1,000-fold, inducing strong and biologically relevant CD8(+) cytotoxic T lymphocyte responses. Because injection directly into a lymph node is a rapid and easy procedure in humans, these results have important clinical implications for DNA vaccination.
尽管DNA疫苗已在动物模型中显示出能引发强烈的免疫反应，但在人体中的初步临床试验结果却令人失望，这凸显了优化其免疫原性的必要性。裸DNA疫苗通常通过肌肉注射或皮内注射给药。当前研究表明，将裸DNA直接注射到外周淋巴结进行免疫接种可使免疫原性提高100至1000倍，诱导强烈且具有生物学相关性的CD8(+)细胞毒性T淋巴细胞反应。由于直接注射到淋巴结在人体中是一种快速且简便的操作，这些结果对DNA疫苗接种具有重要的临床意义。
