Barnett S W, Rajasekar S, Legg H, Doe B, Fuller D H, Haynes J R, Walker C M, Steimer K S
Geniva Inc. Middleton, WI 53562, USA.
Vaccine. 1997 Jun;15(8):869-73. doi: 10.1016/s0264-410x(96)00264-2.
Small animals were immunized with plasmid DNA encoding HIV-1 envelope gp120 either intramuscularly by needle injection (mice and guinea pigs) or epidermally with the Accell gene gun (guinea pits). Subsequently, the animals were boosted with a recombinant gp120 protein subunit vaccine in an oil-in-water based adjuvant, MF59. Antibodies and cytotoxic T-lymphocyte (CTL) immune responses to the HIV envelope glycoprotein were observed in animals immunized with gp120 DNA derived from the HIV-1SF2 laboratory strain or from HIV-1 field isolates. Titers of ELISA antibodies and serum neutralizing antibodies against the HIV-1SF2 laboratory isolate were substantially increased in DNA-immunized animals following a single boost with recombinant gp120 protein subunit. This DNA prime/protein subunit boost immunization approach may be important for vaccination against infectious agents such as HIV for which it is difficult to raise strong antiviral humoral responses with DNA vaccination alone.
小型动物通过针剂肌肉注射（小鼠和豚鼠）或使用Accell基因枪进行表皮免疫接种，以编码HIV-1包膜糖蛋白gp120的质粒DNA进行免疫。随后，用基于水包油佐剂MF59的重组gp120蛋白亚单位疫苗对动物进行加强免疫。在用源自HIV-1SF2实验室毒株或HIV-1野外分离株的gp120 DNA免疫的动物中，观察到了针对HIV包膜糖蛋白的抗体和细胞毒性T淋巴细胞（CTL）免疫反应。在用重组gp120蛋白亚单位进行单次加强免疫后，DNA免疫动物中针对HIV-1SF2实验室分离株的ELISA抗体和血清中和抗体滴度大幅提高。这种DNA初免/蛋白亚单位加强免疫方法对于针对如HIV等感染因子的疫苗接种可能很重要，因为仅用DNA疫苗很难引发强烈的抗病毒体液反应。
