Hinkula J, Lundholm P, Wahren B
Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden.
Vaccine. 1997 Jun;15(8):874-8. doi: 10.1016/s0264-410x(96)00257-5.
The concept of combining several genes in order to immunize against a microbial agent has been tested. We selected human immunodeficiency virus (HIV) genes that individually have been shown to mediate immune responses against HIV proteins. These proteins were the regulating genes/proteins of HIV-1 rev, tat and nef as well as structural genes for gp160 under the control of rev, and the capsid p24 represented by the larger precursor gene p37. Two findings were of particular interest. The combination of these five gene constructs gave strong reactivity to all of them, compared with previous results using each one in single injections. The intranasal immunization route gave good mucosal reactivity by inducing IgG, IgA and T-cell proliferative responses.
为抵御微生物制剂而组合多个基因的概念已得到验证。我们选择了人类免疫缺陷病毒（HIV）基因，这些基因各自已被证明能介导针对HIV蛋白的免疫反应。这些蛋白包括HIV-1 rev、tat和nef的调节基因/蛋白，以及在rev控制下的gp160结构基因，还有由较大的前体基因p37代表的衣壳p24。有两个发现特别令人感兴趣。与之前单次注射每个基因构建体的结果相比，这五个基因构建体的组合对所有构建体都产生了强烈的反应性。鼻内免疫途径通过诱导IgG、IgA和T细胞增殖反应产生了良好的黏膜反应性。
