Assay for valproic acid and its E-delta2 metabolite in rat plasma by capillary gas chromatography without prior derivatization.

作者信息

Carlin A, Simmons J

机构信息

Biopharmaceutics Research Branch, Center for Drug Evaluation and Research, Food and Drug Administration, Rockville, MD 20850, USA.

出版信息

J Chromatogr B Biomed Sci Appl. 1997 Jun 20;694(1):115-21. doi: 10.1016/s0378-4347(97)00110-2.

DOI:10.1016/s0378-4347(97)00110-2

PMID:9234854

Abstract

A new and improved gas chromatographic assay method for valproic acid and a metabolite, E-delta2 valproic acid, in rat plasma has been developed. The assay has sufficient sensitivity to measure free levels of the parent drug and metabolite. By employing a Stabilwax-DA capillary column, symmetrical chromatographic peaks were obtained without the need for prior derivatization. Standard curves for valproic acid were linear from 0.1 to 640 microg/ml. Standard curves for the metabolite were linear from 0.1 to 556 microg/ml.

摘要