Rapid gas chromatographic assay for monitoring valproic acid and valpromide in plasma.

A gas chromatographic (GC) method for monitoring valproic acid and valpromide in plasma was developed. The procedure involved a single solvent extraction of drugs from acidified plasma samples, followed by a GC injection of the organic phase. This rapid, sensitive, specific, and reproducible method is a key factor in pharmacokinetic and stability studies of valpromide. Pharmacokinetic application of the new GC method is presented by a simultaneous plasma monitoring of valpromide and valproic acid levels obtained after intravenous administration of valpromide to a dog.