Cohenford M A, Godwin T A, Cahn F, Bhandare P, Caputo T A, Rigas B
Digilab Division, Bio-Rad, Cambridge, Massachusetts 02193, USA.
Gynecol Oncol. 1997 Jul;66(1):59-65. doi: 10.1006/gyno.1997.4627.
Fourier-transform infrared (FT-IR) spectra of malignant and dysplastic cervical scrapings were abnormal, as first described in our study of a limited number of samples, where the spectra were evaluated by visual inspection and peak intensity ratios. We have expanded our study to evaluate more cervical conditions, and to analyze the spectra by a chemometric approach (principal component analysis [PCA]). Cervical samples from 436 females were evaluated by FT-IR and Papanicolaou testing; 40/436 spectra were nonanalyzable. The remaining were as follows: normal, 174; malignant, 19; dysplasia, 8; atypia, 113; atrophy, 19; inflammatory, 47; bloody smear, 12; hypocellular, 4. PCA analysis followed by chi2 test revealed that statistically significant frequencies of being predicted malignant by FT-IR were associated with samples diagnosed as malignant (P < 0.0001), and also those diagnosed as "atrophy" (P < 0.001), "atypical with bloody smear" (P < 0.05), "atypical with atrophic pattern" (P < 0.05), and "dysplasia" (P < 0.05). Based on these findings, for the diagnosis of cervical cancer by FT-IR, as defined here, the sensitivity is 79%, the specificity is 77%, the positive predictive value is 15%, and the negative predictive value is 98.6%. Our findings (a) demonstrate the application of a chemometric approach to the study of cervical FT-IR spectra; (b) assess its potential diagnostic role; (c) suggest that atrophic and neoplastic samples share structural features; and (d) suggest that blood may interfere with such spectroscopic evaluation. These findings warrant further evaluation of FT- IR spectroscopy in cervical and other malignancies.
恶性和发育异常的宫颈刮片的傅里叶变换红外（FT-IR）光谱是异常的，正如我们在对有限数量样本的研究中首次描述的那样，在该研究中，光谱通过目视检查和峰强度比进行评估。我们扩大了研究范围，以评估更多的宫颈状况，并通过化学计量学方法（主成分分析[PCA]）分析光谱。对436名女性的宫颈样本进行了FT-IR和巴氏试验评估；40/436份光谱无法分析。其余样本情况如下：正常，174份；恶性，19份；发育异常，8份；非典型，113份；萎缩，19份；炎症，47份；血性涂片，12份；细胞过少，4份。PCA分析后进行卡方检验显示，FT-IR预测为恶性的统计学显著频率与诊断为恶性的样本相关（P < 0.0001），也与诊断为“萎缩”（P < 0.001）、“伴有血性涂片的非典型”（P < 0.05）、“伴有萎缩模式的非典型”（P < 0.05）和“发育异常”（P < 0.05）的样本相关。基于这些发现，对于此处定义的通过FT-IR诊断宫颈癌，敏感性为79%，特异性为77%，阳性预测值为15%，阴性预测值为98.6%。我们的发现（a）证明了化学计量学方法在宫颈FT-IR光谱研究中的应用；（b）评估了其潜在的诊断作用；（c）表明萎缩和肿瘤性样本具有共同的结构特征；（d）表明血液可能会干扰这种光谱评估。这些发现值得对FT-IR光谱在宫颈癌和其他恶性肿瘤中的应用进行进一步评估。
