运用随机扩增多态性DNA（RAPD）和PCR指纹技术对4例白血病患者中发生的多育赛多孢菌（膨胀赛多孢菌）暴发进行基因分型。

Use of random amplification of polymorphic DNA (RAPD) and PCR-fingerprinting for genotyping a Scedosporium prolificans (inflatum) outbreak in four leukemic patients.

作者信息

Ruiz-Díez B, Martín-Díez F, Rodríguez-Tudela J L, Alvárez M, Martínez-Suárez J V

机构信息

Unidad de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Spain.

出版信息

Curr Microbiol. 1997 Sep;35(3):186-90. doi: 10.1007/s002849900236.

DOI:10.1007/s002849900236

PMID:9236303

Abstract

Four isolates of the pathogenic fungus Scedosporium prolificans (inflatum), causing a previously reported nosocomial outbreak in four leukemic patients, were typed by random amplification of polymorphic DNA (RAPD) with two different 10-mer primers and PCR-fingerprinting with the core sequence of phage M13 as a single primer. Both techniques allowed 10 additional clinical isolates of Scedosporium prolificans from different areas of Spain, including Scedosporium prolificans NCPF 2884, to be classified into 10 different molecular types. The four outbreak isolates consisted of three molecular types with two patients sharing a similar strain, and the remaining two patients infected by two different strains.

摘要

病原菌多育赛多孢菌（膨胀赛多孢菌）的4个分离株曾在4例白血病患者中引发了一起此前报道过的医院感染暴发，采用两种不同的10聚体引物通过随机扩增多态性DNA（RAPD）分型，并以噬菌体M13的核心序列作为单一引物进行PCR指纹分析。这两种技术还能将另外10株来自西班牙不同地区的多育赛多孢菌临床分离株（包括多育赛多孢菌NCPF 2884）分为10种不同的分子类型。4株暴发分离株包含3种分子类型，其中2例患者感染的是相似菌株，其余2例患者分别感染了两种不同菌株。