Wang H Y, Ross H M, Ng B, Burt M E
Thoracic Oncology Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Ann Thorac Surg. 1997 Jul;64(1):216-9. doi: 10.1016/s0003-4975(97)00343-3.
A pulmonary tumor model is necessary to study the biology and therapy of lung cancer. Methods to establish a solitary intrapulmonary nodule are not well defined. Two methods for solitary intrapulmonary tumor nodule development in the Fischer rat are described.
Methylcholanthrene-induced sarcoma cell suspensions were introduced into lung parenchyma of Fischer rats via limited thoracotomy and lung puncture, or instilled into a distal airway after tracheal puncture and catheterization. Intrapulmonary tumor location, implantation mortality, procedure length, and animal survival were recorded.
Single pulmonary nodules developed at the implanted position in 100% (n = 320) and 95% (62/65) of animals after direct injection into the pulmonary parenchyma or via tracheal puncture and instillation. Operative mortality was 2% and 5% via lung or tracheal implantation, respectively. Less than 5 minutes was required for each implantation. Mean survival time was 24 +/- 2 and 26 +/- 6 days after lung or tracheal implantation in animals allowed to survive until tumor-induced death.
These easily performed, reproducible methods of establishing solitary intrapulmonary tumors are useful tools for lung cancer research.
为了研究肺癌的生物学特性和治疗方法，建立肺部肿瘤模型是必要的。目前，建立孤立性肺内结节的方法尚未明确界定。本文描述了两种在Fischer大鼠中建立孤立性肺内肿瘤结节的方法。
将甲基胆蒽诱导的肉瘤细胞悬液通过有限开胸和肺穿刺注入Fischer大鼠的肺实质，或在气管穿刺和插管后注入远端气道。记录肺内肿瘤位置、植入死亡率、手术时间和动物存活率。
直接注入肺实质或经气管穿刺注入后，100%（n = 320）和95%（62/65）的动物在植入部位出现单个肺结节。经肺植入和经气管植入的手术死亡率分别为2%和5%。每次植入所需时间少于5分钟。在允许存活直至肿瘤诱导死亡的动物中，经肺植入和经气管植入后的平均存活时间分别为24±2天和26±6天。
这些易于实施、可重复的建立孤立性肺内肿瘤的方法是肺癌研究的有用工具。
