Willins D A, Liu B, Xiang X, Morris N R
Dept. of Pharmacology, UMDNJ-Robert Wood Johnson Medical School, Piscataway 08854-5635, USA.
Mol Gen Genet. 1997 Jun;255(2):194-200. doi: 10.1007/s004380050489.
To identify proteins that interact directly or indirectly with the NUDF protein, which is required for nuclear migration in Aspergillus nidulans, we initiated a screen for extragenic suppressors of the heat-sensitive nudF6 mutation. Suppressor mutations in at least five genes, designated snfA-snfE, caused improved growth and nuclear migration at high temperatures compared to the nudF6 parent. Two snfC mutations mapped near the nudA gene, which encodes the cytoplasmic dyncin heavy chain, and could be repaired by transformation with wild-type nudA DNA, demonstrating that they are mutations in nudA. The snfC mutations are bypass suppressors of nudF and genetic evidence indicated that NUDA and NUDF act in the same nuclear migration pathway. Taken together, our data suggests that NUDF affects nuclear migration by acting on the dynein motor system.
为了鉴定与构巢曲霉核迁移所需的NUDF蛋白直接或间接相互作用的蛋白质，我们启动了一项针对热敏感nudF6突变的基因外抑制子的筛选。至少五个基因（命名为snfA - snfE）中的抑制子突变，与nudF6亲本相比，在高温下导致生长改善和核迁移。两个snfC突变定位于nudA基因附近，该基因编码细胞质动力蛋白重链，并且可以通过用野生型nudA DNA转化来修复，表明它们是nudA中的突变。snfC突变是nudF的旁路抑制子，遗传证据表明NUDA和NUDF在相同的核迁移途径中起作用。综上所述，我们的数据表明NUDF通过作用于动力蛋白运动系统来影响核迁移。
