Marquardt J, Senger H, Miyashita H, Miyachi S, Mörschel E
Fachbereich Biologie/Botanik, Philipps-Universität Marburg, Germany.
FEBS Lett. 1997 Jun 30;410(2-3):428-32. doi: 10.1016/s0014-5793(97)00631-5.
Phycobiliprotein aggregates were isolated from the prokaryote Acaryochloris marina, containing chlorophyll d as major pigment. In the electron microscope the biliprotein aggregates appear as rod-shaped structures of 26.0 x 11.3 nm, composed of four ring-shaped subunits 5.8 nm thick and 11.7 nm in diameter. Spectral data indicate that the aggregates contain two types of biliproteins: phycocyanin and an allophycocyanin-type pigment, with very efficient energy transfer from the phycocyanin- to allophycocyanin-type constituent. The chromophore-binding polypeptides of the pigments have apparent molecular masses of 16.2 and 17.4 kDa. They crossreact with antibodies against phycocyanin and allophycocyanin from a red alga.
藻胆蛋白聚集体是从原核生物滨海蓝藻中分离得到的，其中叶绿素d为主要色素。在电子显微镜下，藻胆蛋白聚集体呈现为26.0×11.3纳米的棒状结构，由四个厚度为5.8纳米、直径为11.7纳米的环形亚基组成。光谱数据表明，这些聚集体包含两种类型的藻胆蛋白：藻蓝蛋白和一种别藻蓝蛋白型色素，并且从藻蓝蛋白成分到别藻蓝蛋白型成分存在非常高效的能量转移。这些色素的发色团结合多肽的表观分子量分别为16.2 kDa和17.4 kDa。它们与来自红藻的藻蓝蛋白和别藻蓝蛋白的抗体发生交叉反应。
