Herbert S, Worlitzsch D, Dassy B, Boutonnier A, Fournier J M, Bellon G, Dalhoff A, Döring G
Department of General and Environmental Hygiene, Hygiene-Institute, University of Tübingen, Germany.
J Infect Dis. 1997 Aug;176(2):431-8. doi: 10.1086/514061.
Staphylococcus aureus capsular polysaccharide type 5 (CP5) expression was investigated in lung tissue and nasal polyps of two cystic fibrosis (CF) patients, in rats, and in vitro using ELISA and IFA. In CF tissues, S. aureus expressed protein A and teichoic acid but only 1%-5% of cells expressed CP5. When rats were challenged with CP5-positive S. aureus in the granuloma pouch model, only 1%-5% of CP5-positive cells were detectable in pouch exudates. CF and pouch isolates, however, reexpressed CP5 (70%-90% of cells) when grown in vitro with air. Addition of > or = 1% CO2 to air or to O2/N2 gas mixtures reduced CP5 expression significantly (P < .001) in a dose-dependent manner (6%-1% CP5-positive cells). The results show that S. aureus does not produce CP5 in CF airways and in rat granuloma pouches and that CO2 is an environmental signal that regulates CP5 expression.
使用酶联免疫吸附测定（ELISA）和间接免疫荧光法（IFA），对两名囊性纤维化（CF）患者的肺组织和鼻息肉、大鼠以及体外环境中的金黄色葡萄球菌5型荚膜多糖（CP5）表达情况进行了研究。在CF组织中，金黄色葡萄球菌表达蛋白A和磷壁酸，但仅有1%-5%的细胞表达CP5。当在肉芽肿袋模型中用CP5阳性的金黄色葡萄球菌攻击大鼠时，在袋渗出物中仅能检测到1%-5%的CP5阳性细胞。然而，CF和袋分离株在体外与空气一起培养时会重新表达CP5（70%-90%的细胞）。向空气或氧气/氮气混合气体中添加≥1%的二氧化碳会以剂量依赖的方式显著降低CP5表达（P<0.001）（CP5阳性细胞从6%降至1%）。结果表明，金黄色葡萄球菌在CF气道和大鼠肉芽肿袋中不产生CP5，并且二氧化碳是调节CP5表达的一种环境信号。
