Daher G C, Cooper D A, Zorich N L, King D, Riccardi K A, Peters J C
The Procter & Gamble Company, Winton Hill Technical Center, Cincinnati, OH 45224, USA.
J Nutr. 1997 Aug;127(8 Suppl):1694S-1698S. doi: 10.1093/jn/127.8.1694S.
The effect of olestra, a zero-calorie fat replacement, on the absorption of dietary fat was determined with a dual-isotope technique in 67 healthy male subjects. After a 30-d adaptation period in which they consumed potato chips which delivered either 10 g/d olestra or 10 g/d triglyceride under free-living conditions, the subjects were housed in a metabolic ward and given 0, 8, 20 or 32 g olestra in potato chips. The chips were eaten as part of a breakfast containing about 38 g of fat, about 0.16 mg of 14C-triolein, and a nonabsorbable marker, 51CrCl3. Feces were collected for 7 d, and aliquots of the two daily collections containing the highest levels of 51Cr were oxidized. The CO2 was collected, and 14C content was determined by liquid scintillation spectrometry. The fractional absorption of 14C-triolein was calculated from the average ratios of 14C/51Cr dosed and measured in the feces. Olestra had a slight but significant dose-response effect on triglyceride absorption: the highest olestra dose (32 g) reduced absorption by 1.2%. This effect is not nutritionally significant with respect to either availability of essential fatty acids or energy intake.
采用双同位素技术在67名健康男性受试者中测定了零热量脂肪替代品蔗糖聚酯对膳食脂肪吸收的影响。在30天的适应期内，受试者在自由生活条件下食用每天提供10克蔗糖聚酯或10克甘油三酯的薯片，之后将他们安置在代谢病房，并给予他们含有0、8、20或32克蔗糖聚酯的薯片。这些薯片作为早餐的一部分食用，早餐含有约38克脂肪、约0.16毫克14C-三油酸甘油酯以及一种不可吸收的标记物51CrCl3。收集粪便7天，将每天两次收集的含有最高水平51Cr的等分试样进行氧化。收集二氧化碳，通过液体闪烁光谱法测定14C含量。根据粪便中给药和测量的14C/51Cr平均比值计算14C-三油酸甘油酯的吸收分数。蔗糖聚酯对甘油三酯吸收有轻微但显著的剂量反应效应：最高剂量的蔗糖聚酯（32克）使吸收降低了1.2%。就必需脂肪酸的可利用性或能量摄入而言，这种效应在营养方面并不显著。
