Makrigiannakis A, Psychoyos A, Zoumakis E, Margioris A N, Stournaras C, Gravanis A
Department of Pharmacology, Medical School, University of Crete Iraklion, Greece.
Ann N Y Acad Sci. 1997 Jun 17;816:116-28. doi: 10.1111/j.1749-6632.1997.tb52135.x.
Our findings show that human and rat uterus express the CRH gene. Epithelial cells of both species are the main source of endometrial CRH, while stroma does not seem to express it, unless it differentiates to decidua. Immunoreactive CRH, produced by endometrial cells, has the chromatographic characteristics of authentic hypothalamic CRH, while the size of its mRNA in both human and rat uterus is similar to or identical with its counterpart, present in placenta and hypothalamus (1.3 kb). Estrogens and glucocorticoids inhibit and prostaglandin E2 stimulates the promoter of human CRH gene in transfected human endometrial cells, suggesting that endometrial CRH gene expression is under the control of these agents. Moreover, in rats, endometrial CRH expression is significantly higher at implantation sites, compared to that at interimplantation uterine regions. Given the proinflammatory/vasoregulatory properties of CRH, we hypothesize that endometrial CRH may participate in the regulation of intrauterine phenomena, such as blastocyst implantation, endometrial vascularization, and myometrial contractility.
我们的研究结果表明，人类和大鼠子宫均表达促肾上腺皮质激素释放激素（CRH）基因。两种物种的上皮细胞均为子宫内膜CRH的主要来源，而基质细胞似乎不表达，除非其分化为蜕膜。子宫内膜细胞产生的免疫反应性CRH具有真正下丘脑CRH的色谱特征，而在人类和大鼠子宫中其mRNA的大小与其在胎盘和下丘脑中的对应物相似或相同（1.3kb）。雌激素和糖皮质激素抑制而前列腺素E2刺激转染的人类子宫内膜细胞中人类CRH基因的启动子，提示子宫内膜CRH基因表达受这些因子调控。此外，在大鼠中，与子宫非着床部位相比，着床部位的子宫内膜CRH表达显著更高。鉴于CRH的促炎/血管调节特性，我们推测子宫内膜CRH可能参与子宫内现象的调节，如胚泡着床、子宫内膜血管形成和子宫肌层收缩。
