Sackman J E, Daniel G B, Freeman M B, Lothrop C D
College of Veterinary Medicine, Department of Small Animal Clinical Sciences, University Medical Center, University of Tennessee; Knoxville 37920, USA.
Vet Radiol Ultrasound. 1997 Mar-Apr;38(2):150-5. doi: 10.1111/j.1740-8261.1997.tb00830.x.
Endothelial seeding of vascular grafts has been shown to decrease graft thrombogenicity and prolong longevity when implanted in vivo. Previous studies have utilized anatomic grafts to study endothelialization and healing. Anatomic thoracoabdominal grafts do not allow for sequential biopsy for evaluation of individual grafts nor do they approximate the environment for long bypass grafts used in limb salvage. This study evaluated the use of an extra-anatomic aortic bypass graft to assess the healing of endothelial cell seeded expanded polytetrafluoroethylene (ePTFE). Radionuclide angiography was used to evaluate graft patency and quantify blood flow through the graft. Dogs underwent placement of an extra-anatomic 60 cm long, 8 mm internal diameter, graft seeded with autologous endothelium. Grafts were biopsied from 2 weeks up to 1 year. Radionuclide studies were performed postimplantation and following each graft biopsy. Graft placement and biopsies were well tolerated in all dogs. Biopsied segments of graft allowed for sequential studies of the healing of implanted grafts by scanning electron and light microscopy. Flow through the implanted graft was close to 50% of the total caudal abdominal aortic flow. No significant difference in graft flow was noted either between animals or over time.
血管移植物的内皮细胞种植已被证明，当植入体内时可降低移植物的血栓形成性并延长其使用寿命。先前的研究利用解剖学移植物来研究内皮化和愈合情况。解剖学胸腹移植物既不允许对单个移植物进行连续活检以进行评估，也无法模拟用于肢体挽救的长旁路移植物的环境。本研究评估了使用解剖外主动脉旁路移植物来评估内皮细胞种植的膨体聚四氟乙烯（ePTFE）的愈合情况。放射性核素血管造影用于评估移植物通畅情况并量化通过移植物的血流量。对犬进行解剖外60厘米长、内径8毫米、种植有自体内皮的移植物植入。从2周到1年对移植物进行活检。在植入后以及每次移植物活检后进行放射性核素研究。所有犬对移植物植入和活检的耐受性良好。通过扫描电子显微镜和光学显微镜对移植物的活检切片进行连续研究，以观察植入移植物的愈合情况。通过植入移植物的血流量接近腹主动脉总尾端血流量的50%。在不同动物之间或随时间推移，移植物血流量均未发现显著差异。
