Seeger J M, Klingman N
Department of Surgery, University of Florida, Gainesville.
J Vasc Surg. 1988 Oct;8(4):476-82. doi: 10.1067/mva.1988.avs0080476.
Endothelial cell growth in vitro is enhanced by coating with fibronectin the surface on which cells grow. Similar coating of prosthetic arterial grafts may promote in vivo graft endothelialization if graft patency is not adversely affected. In each of 15 dogs, two fibronectin-coated polytetrafluoroethylene grafts and two grafts that were not coated were implanted. One graft in each pair was seeded with autologous endothelial cells, so that four different grafts were studied in each animal: a coated, seeded graft; a coated graft that was not seeded; a seeded graft that was not coated; a graft that was neither coated nor seeded. At 2, 4, and 8 weeks, grafts from five animals were examined for patency, surface endothelialization, and indium 111 platelet reactivity. After seeding, surface coverage by endothelium of coated grafts was more complete and more rapid than in uncoated grafts (64% +/- 23% vs 31% +/- 13% at 4 weeks, p less than 0.05). Without seeding, coated grafts also appeared to have increased endothelial cell ingrowth compared with plain grafts (48.8% +/- 15.1% vs 37.6% +/- 1.5% at 8 weeks). Early (2-week) platelet reactivity of coated grafts was increased (p = 0.06), but patency was not adversely affected. Thus fibronectin coating of prosthetic grafts promotes surface endothelialization in vivo without altering graft patency.
通过在细胞生长的表面包被纤连蛋白，可增强内皮细胞的体外生长。如果人工动脉移植物的通畅性不受不利影响，对其进行类似的包被可能会促进体内移植物的内皮化。在15只狗中，每只狗都植入了两根包被纤连蛋白的聚四氟乙烯移植物和两根未包被的移植物。每对移植物中的一根接种自体内皮细胞，因此在每只动物中研究了四种不同的移植物：包被且接种的移植物；包被但未接种的移植物；接种但未包被的移植物；既未包被也未接种的移植物。在第2、4和8周时，对来自5只动物的移植物进行通畅性、表面内皮化和铟111血小板反应性检查。接种后，包被移植物的内皮表面覆盖比未包被移植物更完整、更迅速（4周时为64%±23%对31%±13%，p<0.05）。未接种时，与普通移植物相比，包被移植物的内皮细胞向内生长也似乎增加（8周时为48.8%±15.1%对37.6%±1.5%）。包被移植物的早期（2周）血小板反应性增加（p = 0.06），但通畅性未受不利影响。因此，人工移植物的纤连蛋白包被可促进体内表面内皮化，而不改变移植物的通畅性。
