Carrillo de Padilla F
Cátedra de Análisis de Alimentos, Facultad de Farmacia UCV, Caracas, Venezuela.
Arch Latinoam Nutr. 1996 Jun;46(2):169-73.
The separation and identification by HPLC of the cis isomers of beta-carotene was studied. A 1.26 mg/ml beta-carotene solution previously isomerized with iodine as a catalyst, was eluted with 2% acetone in hexane, from a Ca(OH)2 chromatographic column in three bands. The fractions were identified by spectrophotometry and the retention times of 2.05, 2.4 and 2.8 min for the 13 cis, all-trans, and 9 cis beta-carotene isomers, determined by HPLC, with 1% acetone in hexane as movil phase. 22.13 mg % of all-trans beta-carotene were found in a sample of canned carrots. It is recommended the analyses of a greater number of samples, the determination of the method's sensitivity, reproductibility, and the use of a standard of reference of a response factor for calculations.
研究了用高效液相色谱法（HPLC）分离和鉴定β-胡萝卜素顺式异构体。将先前以碘为催化剂异构化的1.26mg/mlβ-胡萝卜素溶液，用2%丙酮的己烷溶液从Ca(OH)₂色谱柱上洗脱，得到三个谱带。通过分光光度法对馏分进行鉴定，并以1%丙酮的己烷溶液为流动相，用HPLC测定了13-顺式、全反式和9-顺式β-胡萝卜素异构体的保留时间分别为2.05、2.4和2.8分钟。在罐装胡萝卜样品中发现全反式β-胡萝卜素含量为22.13mg%。建议分析更多数量的样品，确定该方法的灵敏度、重现性，并使用响应因子的标准参考物进行计算。
