一种用于同时分离虾青素主要顺式/反式构型异构体的手性高效液相色谱法。

A chiral HPLC method for the simultaneous separation of configurational isomers of the predominant cis/trans forms of astaxanthin.

作者信息

Abu-Lafi S, Turujman S A

机构信息

Office of Cosmetics and Colors, U.S. Food and Drug Administration, Washington, DC 20204, USA.

出版信息

Enantiomer. 1997;2(1):17-25.

PMID:9676271

Abstract

We report an HPLC method that allows the simultaneous separation of configurational isomers of the predominant cis/trans forms of astaxanthin. The configurational isomers of the all-trans-, and most of the configurational isomers of the 9-cis-, 13-cis- and 15-cis-astaxanthin were separated on a Sumichiral OA-2000 column, which is manufactured and packed in Japan with a Pirkle covalent D-phenylglycine chiral stationary phase (CSP). The large separation of the cis isomers from the all-trans isomers that we report here ensure the suitability of this method for the routine determination of the ratio of the configurational isomers of all-trans-astaxanthin.

摘要

我们报道了一种高效液相色谱（HPLC）方法，该方法能够同时分离虾青素主要顺式/反式构型异构体。全反式虾青素的构型异构体以及9-顺式、13-顺式和15-顺式虾青素的大部分构型异构体在Sumichiral OA - 2000柱上实现了分离，该柱在日本制造并装填了具有Pirkle共价D - 苯甘氨酸手性固定相（CSP）。我们在此报道的顺式异构体与全反式异构体之间的较大分离度确保了该方法适用于常规测定全反式虾青素构型异构体的比例。