Murakoshi M, Ikeda R, Tagawa M, Takekoshi S, Watanabe K
Safety Research Department, Teikoku Hormone Mfg. Co., Ltd., Kawasaki, Japan.
Tokai J Exp Clin Med. 1996 Jun;21(2):55-9.
Immunolocalization of glutathione-peroxidase (GSH-PO) in the rat ventral prostate was studied in the presence and absence of androgen. Male Sprague-Dawley rats were divided into four experimental groups. Group 1 consisted of intact controls. In group 2, rats were sacrificed two days after castration. In groups 3 and 4, rats were injected subcutaneously with 1 mg of testosterone-propionate daily, for three or seven days, beginning two days after castration. The intensity of GSH-PO staining in the glandular epithelial cells of the ventral prostate decreased after castration, but recovered following treatment with testosterone. Furthermore, the prostatic GSH-PO mRNA levels were diminished in the castrated rat ventral prostate but greatly increased by testosterone. These findings strongly suggest that the expression of GSH-PO in the glandular epithelial cells of the rat ventral prostate is dependent on testosterone.
在有雄激素和无雄激素的情况下，研究了大鼠腹侧前列腺中谷胱甘肽过氧化物酶（GSH-PO）的免疫定位。雄性斯普拉格-道利大鼠被分为四个实验组。第1组为完整对照组。第2组大鼠在去势两天后处死。在第3组和第4组中，大鼠在去势两天后开始每天皮下注射1毫克丙酸睾酮，持续三天或七天。去势后腹侧前列腺腺上皮细胞中GSH-PO染色强度降低，但用睾酮治疗后恢复。此外，去势大鼠腹侧前列腺中前列腺GSH-PO mRNA水平降低，但睾酮使其大幅增加。这些发现有力地表明，大鼠腹侧前列腺腺上皮细胞中GSH-PO的表达依赖于睾酮。
