Dewey R A, Semorile L C, Grau O, Crisci J V
Instituto de Bioquímica y Biología Molecular, Fac. Cs. Exactas, Universidad Nacional de La Plata, Argentina.
Mol Phylogenet Evol. 1997 Aug;8(1):11-32. doi: 10.1006/mpev.1996.0401.
The genus Tospovirus was thought to be composed only of the tomato spotted wilt virus (TSWV), but now at least four Tospovirus species have been proposed based on serological and molecular data. A classification of tospoviruses has been proposed taking into account global similarities of the N gene and N protein sequences of 7 isolates of Tospovirus. Because phylogenetic analyses based on global similarities can lead to classifications which do not mirror the genealogy of the group, we have employed a cladistic analysis using parsimony of this genus with RNA sequences of 450 nucleotides of the N gene from 14 new Argentinean isolates and 4 previously described isolates. Representatives of the Bunyaviridae family, Rift Valley Fever Virus (Phlebovirus) and Bunyamwera (Bunyavirus), were used as the outgroup in separate analyses.
番茄斑萎病毒属曾被认为仅由番茄斑萎病毒（TSWV）构成，但现在基于血清学和分子数据已提出至少四种番茄斑萎病毒属病毒。已提出一种番茄斑萎病毒属病毒的分类方法，该方法考虑了番茄斑萎病毒属7个分离株的N基因和N蛋白序列的全球相似性。由于基于全球相似性的系统发育分析可能导致分类结果无法反映该类群的谱系，因此我们采用了简约分支分析方法，对来自14个新的阿根廷分离株和4个先前描述的分离株的N基因450个核苷酸的RNA序列进行了该属的分析。在单独的分析中，布尼亚病毒科的代表裂谷热病毒（白蛉病毒属）和布尼亚姆韦拉病毒（布尼亚病毒属）被用作外类群。
