Universidad Autónoma de Santo Domingo-UASD and Instituto Dominicano de Investigaciones Agropecuarias y Florestales - IDIAF, Santo Domingo, Dominican Republic.
Departamento de Biologia Celular, Universidade de Brasília, Brasilia, Brazil.
Virol J. 2018 Jan 26;15(1):24. doi: 10.1186/s12985-018-0931-9.
The Tomato chlorotic spot virus (TCSV) was first reported in the 1980s, having its occurrence limited to Brazil and Argentina. Due to an apparent mild severity in the past, molecular studies concerning TCSV were neglected. However, TCSV has disseminated over the USA and Caribbean countries. In Dominican Republic TCSV has been recently reported on important cultivated crops such as pepper and beans. In this work, we provide the first complete genome of a TCSV isolate from symptomatic plants in Dominican Republic, which was obtained by high-throughput sequencing. In addition, three dsRNA viruses from different virus families were identified coinfecting these plants Bell pepper endornavirus (BPEV), Southern tomato virus (STV) and Pepper cryptic virus 2 (PCV-2). Phylogenetic analysis showed that the Dominican Republic TCSV isolate has a close relationship with other TCSV isolates and a reassortant isolate between TCSV and Groundnut ringspot virus (GRSV), all found in USA. BPEV, STV and PCV-2 isolates from Dominican Republic were close related to corresponding American isolates. The possible biological implications of these virus-mixed infections are discussed.
番茄褪绿斑点病毒（TCSV）于 20 世纪 80 年代首次报道，其发生范围仅限于巴西和阿根廷。由于过去的严重程度明显较轻，因此忽视了有关 TCSV 的分子研究。然而，TCSV 已经传播到美国和加勒比国家。在多米尼加共和国，TCSV 最近在辣椒和豆类等重要栽培作物上有报道。在这项工作中，我们提供了来自多米尼加共和国有症状植物的 TCSV 分离株的第一个完整基因组，该分离株是通过高通量测序获得的。此外，还鉴定了三种来自不同病毒科的 dsRNA 病毒同时感染这些植物：辣椒内胚病毒（BPEV）、南方番茄病毒（STV）和胡椒隐性病毒 2（PCV-2）。系统发育分析表明，多米尼加共和国的 TCSV 分离株与其他 TCSV 分离株以及美国发现的 TCSV 和花生环斑病毒（GRSV）之间的重组分离株关系密切。来自多米尼加共和国的 BPEV、STV 和 PCV-2 分离株与相应的美国分离株密切相关。讨论了这些病毒混合感染的可能生物学意义。
