Wold M S
Department of Biochemistry, University of Iowa College of Medicine, Iowa City 52242, USA.
Annu Rev Biochem. 1997;66:61-92. doi: 10.1146/annurev.biochem.66.1.61.
Replication protein A [RPA; also known as replication factor A (RFA) and human single-stranded DNA-binding protein] is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism, including DNA replication, DNA repair, and recombination. RPA homologues have been identified in all eukaryotic organisms examined and are all abundant heterotrimeric proteins composed of subunits of approximately 70, 30, and 14 kDa. Members of this family bind nonspecifically to single-stranded DNA and interact with and/or modify the activities of multiple proteins. In cells, RPA is phosphorylated by DNA-dependent protein kinase when RPA is bound to single-stranded DNA (during S phase and after DNA damage). Phosphorylation of RPA may play a role in coordinating DNA metabolism in the cell. RPA may also have a role in modulating gene expression.
复制蛋白A [RPA；也称为复制因子A (RFA) 和人单链DNA结合蛋白] 是一种单链DNA结合蛋白，参与真核生物DNA代谢的多个过程，包括DNA复制、DNA修复和重组。在所研究的所有真核生物中均已鉴定出RPA同源物，它们都是由约70、30和14 kDa亚基组成的丰富的异源三聚体蛋白。该家族成员非特异性地结合单链DNA，并与多种蛋白质相互作用和/或改变其活性。在细胞中，当RPA与单链DNA结合时（在S期和DNA损伤后），会被DNA依赖性蛋白激酶磷酸化。RPA的磷酸化可能在协调细胞中的DNA代谢中发挥作用。RPA也可能在调节基因表达中起作用。
