Iwatsuki H, Sasaki K, Suda M, Itano C
Department of Anatomy, Kawasaki Medical School, Kurashiki, Japan.
Histochem Cell Biol. 1997 Jun;107(6):459-68. doi: 10.1007/s004180050133.
To clarify the origin of the central cells in hepatic erythroblastic islands, glycoconjugates on the surface of cellular constituents in fetal mice liver were ultrahistochemically examined using lectin staining. At 11 days of gestation, the cells derived from mesenchyme in fetal liver, including sinusoidal macrophages, endothelial cells, and erythropoietic cells, bound Griffonia simplicifolia isoagglutinin I-B4 (GS-I-B4), but hepatocytes lacked binding sites for the isolectin. Scavenger macrophages in the hepatic cords at 13 days of gestation and the central cells in the erythroblastic islands at 15 days of gestation also bound GS-I-B4. Hepatocytes, however, exhibited no GS-I-B4 binding site at any gestational day. At 11 days of gestation, none of the cells in fetal liver had binding sites for soybean agglutinin (SBA), but cells derived from mesenchyme acquired these binding sites at 13 days of gestation. The central cells in the erythroblastic islands also bound SBA, but hepatocytes did not bind the lectin at all. The central cells in the erythroblastic islands can be considered to belong to a mesenchymal cell lineage, and primitive sinusoidal macrophages at 11 days of gestation are possible precursors of these central cells.
为阐明肝内成红细胞岛中央细胞的起源，利用凝集素染色对胎鼠肝脏细胞成分表面的糖缀合物进行了超微组织化学检查。在妊娠11天时，胎肝中来源于间充质的细胞，包括窦周巨噬细胞、内皮细胞和造血细胞，能与简单型非洲豆蔻凝集素I-B4（GS-I-B4）结合，但肝细胞缺乏该异凝集素的结合位点。妊娠13天时肝索中的清道夫巨噬细胞以及妊娠15天时成红细胞岛中的中央细胞也能与GS-I-B4结合。然而，肝细胞在任何妊娠日都未表现出GS-I-B4结合位点。在妊娠11天时，胎肝中的细胞均无大豆凝集素（SBA）的结合位点，但来源于间充质的细胞在妊娠13天时获得了这些结合位点。成红细胞岛中的中央细胞也能与SBA结合，但肝细胞完全不与该凝集素结合。成红细胞岛中的中央细胞可被认为属于间充质细胞谱系，妊娠11天时的原始窦周巨噬细胞可能是这些中央细胞的前体。
