Iwatsuki H, Sasaki K, Suda M, Itano C
Department of Anatomy, Kawasaki Medical School, Kurashiki, Japan.
Histochemistry. 1993 Nov;100(5):331-40. doi: 10.1007/BF00268931.
Glycoconjugates on the surface of pulmonary epithelial cells were ultrahistochemically examined in the fetal, neonatal and adult rat lung. Lectin and colloidal iron staining procedures were performed in combination with digestion using carbohydrate-degrading enzymes or methylation. The glycoconjugate composition of columnar cells at 16 days gestation was similar to that of cuboidal cells at 19 days gestation. Glycoconjugate differentiation on the cell surface occurred at 20 days gestation, and especially the loss of soybean agglutinin (SBA) binding sites could be detected on type II cells. The contents of Ricinus communis agglutinin-I (RCA-I) and Concanavalin A (Con A) binding sites on type II cells also began to decrease. On the contrary, the content of sulfated saccharides decreased on the surface of type I cells during development. Glycoconjugate differentiation on both type I and II cells was completed with the disappearance of hyaluronic acid and peanut agglutinin (PNA) binding sites; type I and II cells acquired a similar histochemical composition to that on adult type I and II cells at 5 days after birth. Both type I and II cells share a common early precursor cell, that is, the cuboidal epithelial cell at the canalicular stage.
采用超微组织化学方法，对胎鼠、新生鼠和成年大鼠肺脏的肺上皮细胞表面糖缀合物进行了检测。结合使用碳水化合物降解酶或甲基化进行消化，开展了凝集素和胶体铁染色程序。妊娠16天时柱状细胞的糖缀合物组成与妊娠19天时立方体细胞的相似。细胞表面的糖缀合物分化发生在妊娠20天时，尤其在II型细胞上可检测到大豆凝集素（SBA）结合位点的丧失。II型细胞上蓖麻凝集素-I（RCA-I）和伴刀豆球蛋白A（Con A）结合位点的含量也开始减少。相反，在发育过程中I型细胞表面硫酸化糖类的含量减少。随着透明质酸和花生凝集素（PNA）结合位点的消失，I型和II型细胞上的糖缀合物分化完成；出生后5天时，I型和II型细胞获得了与成年I型和II型细胞相似的组织化学组成。I型和II型细胞均源自共同的早期前体细胞，即小管期的立方上皮细胞。
