Möller T, Kann O, Prinz M, Kirchhoff F, Verkhratsky A, Kettenmann H
Max-Delbrück-Center for Molecular Medicine, Berlin-Buch, Germany.
Neuroreport. 1997 Jul 7;8(9-10):2127-31. doi: 10.1097/00001756-199707070-00008.
Microglial cells are the intrinsic immunocompetent cells of the central nervous system, which are activated by brain tissue damage. In this paper we investigated the ability of endothelins (ETs), which are potent vasoconstrictors, to induce intracellular calcium signals in cultured microglia cells. Both endothelin-1 and endothelin-3 increased intracellular Ca2+ concentration ([Ca2+]i). These [Ca2+]i transients were mimicked by BQ3020, an ETB receptor agonist and blocked by BQ788, a selective ETB antagonist, respectively. The calcium signals induced by the endothelins persisted in Ca(2+)-free media. Transcripts encoding the ETB receptor were detected in purified microglial cultures and cDNA fragments derived from ETB receptor mRNA were amplified from 9% of electrophysiologically characterized microglial cells by the use of single-cell RT-PCR.
小胶质细胞是中枢神经系统固有的免疫活性细胞，可被脑组织损伤激活。在本文中，我们研究了强效血管收缩剂内皮素（ETs）在培养的小胶质细胞中诱导细胞内钙信号的能力。内皮素-1和内皮素-3均增加了细胞内Ca2+浓度（[Ca2+]i）。这些[Ca2+]i瞬变分别被ETB受体激动剂BQ3020模拟，并被选择性ETB拮抗剂BQ788阻断。内皮素诱导的钙信号在无钙培养基中持续存在。在纯化的小胶质细胞培养物中检测到编码ETB受体的转录本，并且通过单细胞逆转录聚合酶链反应从9%的经电生理特征鉴定的小胶质细胞中扩增出源自ETB受体mRNA的cDNA片段。
