Hirose Akira, Azuma Hiroshi, Tokoro Takashi, Hirai Keiji
Department of Ophthalmology and Visual Science, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
Curr Eye Res. 2007 Jun;32(6):585-91. doi: 10.1080/02713680701391529.
The physiological properties of endothelin-1 in ocular choroidal melanocytes are not well-known, although endothelin-1 increases the intracellular calcium concentration through endothelin-B receptors in skin melanocytes. We studied the effect of endothelin-1 on choroidal melanocytes in rabbit ocular suprachoroidal tissue. Fura-2 microfluorophotometry indicated that endothelin-1 and endothelin-B receptor selective agonists, endothelin-3 and BQ3020, increased the intracellular calcium concentration of the tissue. Endothelin-B receptor selective antagonist BQ788 blunted the response. Immuno-electron microscopy showed that endothelin-B receptor-like immunoreactivity was located only on melanocytes. These results indicate that endothelin-1 increases intracellular calcium concentration in rabbit suprachoroidal tissue via endothelin-B receptors on melanocytes.
内皮素-1在眼脉络膜黑素细胞中的生理特性尚不清楚，尽管内皮素-1通过皮肤黑素细胞中的内皮素B受体增加细胞内钙浓度。我们研究了内皮素-1对兔眼脉络膜上组织中脉络膜黑素细胞的影响。Fura-2显微荧光测定法表明，内皮素-1以及内皮素B受体选择性激动剂内皮素-3和BQ3020可增加该组织的细胞内钙浓度。内皮素B受体选择性拮抗剂BQ788可减弱这种反应。免疫电子显微镜显示，内皮素B受体样免疫反应仅位于黑素细胞上。这些结果表明，内皮素-1通过黑素细胞上的内皮素B受体增加兔脉络膜上组织中的细胞内钙浓度。
