缺氧和电刺激对沙蠋体壁肌肉组织中ATP、ADP、AMP和磷酸牛磺酸浓度的影响。

The influence of oxygen deficiency and electrical stimulation on the concentrations of ATP, ADP, AMP and phosphotaurocyamine in the body-wall musculature of Arenicola marina.

Surholt B

Hoppe Seylers Z Physiol Chem. 1977 Nov;358(11):1455-61. doi: 10.1515/bchm2.1977.358.2.1455.

Abstract

Concentrations of ATP, ADP, and AMP, as well as phosphotaurocyamine in the body-wall musculature of Arenicola marina were determined enzymatically and by means of isotachophoresis after 12, 24, and 48 h of experimental anaerobiosis and after electrical stimulation under aerobic and anaerobic conditions. 2) Freshly collected lugworms contained 3.5-3.8 mumol ATP, 0.8-1.0 mumol ADP, and 0.3-0.5 mumol AMP, as well as 4.5-4.7 mumol phosphotaurocyamine per g wet weight (energy charge 0.81-0.85). 3) After 48 h of anaerobiosis the concentration of ATP was decreased to one half, that of phosphotaurocyamine to one third of the initial value, while the content of ADP on the other hand nearly doubled and that of AMP trebled (energy charge 0.60-0.63). 4) Electrical stimulation in the absence of oxygen caused a rapid decrease of the ATP and phosphotaurocyamine to low levels corresponding to those found after long-term anaerobiosis; the ADP and AMP concentrations were slightly higher than those found after long-term anaerobiosis (energy charge 0.55-0.58).

摘要

在实验性无氧状态12、24和48小时后，以及在有氧和无氧条件下进行电刺激后，采用酶法和等速电泳法测定了沙蠋体壁肌肉组织中ATP、ADP、AMP以及磷酸氨基乙磺酸的浓度。2) 刚采集的沙蠋每克湿重含有3.5 - 3.8微摩尔ATP、0.8 - 1.0微摩尔ADP、0.3 - 0.5微摩尔AMP，以及4.5 - 4.7微摩尔磷酸氨基乙磺酸（能荷为0.81 - 0.85）。3) 无氧状态48小时后，ATP浓度降至初始值的一半，磷酸氨基乙磺酸浓度降至初始值的三分之一，而另一方面，ADP含量几乎翻倍，AMP含量增至三倍（能荷为0.60 - 0.63）。4) 在无氧条件下进行电刺激会使ATP和磷酸氨基乙磺酸迅速降至与长期无氧状态后相当的低水平；ADP和AMP浓度略高于长期无氧状态后的浓度（能荷为0.55 - 0.58）。