Spriet L L, Söderlund K, Bergström M, Hultman E
J Appl Physiol (1985). 1987 Feb;62(2):611-5. doi: 10.1152/jappl.1987.62.2.611.
The quadriceps femoris muscles of seven men were electrically stimulated under extended anaerobic conditions to quantitate anaerobic energy release and the contribution of the glycolytic system to total ATP production. Muscles were intermittently stimulated 64 times at 20 Hz while leg blood flow was occluded. Each contraction lasted 1.6 s and was followed by 1.6 s of rest. The total contraction time was 102.4 s. Muscle biopsies were taken at rest and following 16, 32, 48, and 64 contractions. The ATP turnover rates during the four 16-contraction periods were 6.12, 2.56, 2.17, and 0.64 mmol X kg dry muscle-1 X s-1 contraction time. Glycolysis provided 58%, phosphocreatine 40% and a decreased ATP store 2% of the consumed energy during the initial 16 contractions. Glycolysis was responsible for 90% of the total ATP production beyond contraction 16. Absolute glycolytic ATP production decreased to 60, 55, and 17% of the amount in the initial 16 contractions during the final three periods, respectively. In conclusion glycolysis produced approximately 195 mmol ATP/kg dry muscle during the initial 48 contractions (76.8 s) and only approximately 15 mmol ATP/kg dry muscle during the final 16 contractions. Equivalent values for total ATP turnover were 278 and 16.5 mmol/kg dry muscle.
在无氧条件下对7名男性的股四头肌进行电刺激，以定量无氧能量释放以及糖酵解系统对总ATP生成的贡献。在阻断腿部血流的情况下，以20Hz的频率对肌肉进行64次间歇性刺激。每次收缩持续1.6秒，随后休息1.6秒。总收缩时间为102.4秒。在休息时以及进行16、32、48和64次收缩后采集肌肉活检样本。在四个16次收缩期间的ATP转换率分别为6.12、2.56、2.17和0.64 mmol·kg干肌肉-1·s-1收缩时间。在最初的16次收缩中，糖酵解提供了58%的消耗能量，磷酸肌酸提供了40%，ATP储备减少提供了2%。在第16次收缩之后，糖酵解负责总ATP生成的90%。在最后三个时期，绝对糖酵解ATP生成分别降至最初16次收缩时生成量的60%、55%和17%。总之，在最初的48次收缩（76.8秒）期间，糖酵解产生了约195 mmol ATP/kg干肌肉，而在最后的16次收缩期间仅产生了约15 mmol ATP/kg干肌肉。总ATP转换的等效值分别为278和16.5 mmol/kg干肌肉。
