Kitano Y, Yasuda N, Shimizu T, Ohzono H, Iwamoto T
Laboratory of Veterinary Pathology, Faculty of Agriculture, Kagoshima University, Japan.
Res Vet Sci. 1997 Mar-Apr;62(2):195-8. doi: 10.1016/s0034-5288(97)90147-8.
Aino virus (JaNAr 28 strain), a possible agent of a congenital anomaly of calves, was inoculated into the yolk sac of chick embryos at a dose of 10(2), 10(3), 10(4) or 10(5) TCID50 0.2 ml-1 at four, six or eight days of incubation. At 21 days of incubation all the unhatched embryos or hatched chickens in the experimental and control groups were sacrificed for pathological examination. The incidence of hydranencephaly, cerebellar hypoplasia or agenesis and arthrogryposis or scoliosis was highest (85 per cent in the chicks inoculated with 10(3) TCID50 0.2 ml-1 at eight days of incubation. The lesions were very similar to those found in congenital abnormalities in calves suspected of a natural infection with Aino virus.
爱诺病毒（JaNAr 28株），一种可能导致犊牛先天性异常的病原体，于孵化的第4、6或8天，以10(2)、10(3)、10(4)或10(5) TCID50 0.2 ml-1的剂量接种到鸡胚的卵黄囊中。在孵化21天时，对实验组和对照组中所有未孵化的胚胎或已孵化的鸡进行宰杀以进行病理检查。积水性无脑、小脑发育不全或发育不全以及关节弯曲或脊柱侧弯的发生率最高（在孵化第8天接种10(3) TCID50 0.2 ml-1的雏鸡中为85%）。这些病变与怀疑自然感染爱诺病毒的犊牛先天性异常中发现的病变非常相似。
