Darke C, Street J, Fussell H, Thomas M, Guttridge M, Goldberg T E, Arnett K L, Parham P
Regional Tissue Typing Laboratory, Welsh Blood Service, Wales, United Kingdom.
Tissue Antigens. 1997 Jul;50(1):32-7. doi: 10.1111/j.1399-0039.1997.tb02830.x.
Using HLA serology, we detected a new variant of HLA-B44- B44BO- in two families. This antigen reacts with B44 antisera and is negative with over one-third of B12 (B44, B45) sera but reacts with 50% antisera with a B62 component, especially if they contain anti-B57. The variant, B4408, differs from the common B4402 by 4 nucleotide substitutions in exon 2: 193, 206 and 209, which produce changes in the the alpha 1 domain at positions 41, 45 and 46 (TKE in B4402 and AMA in B44BO); and nucleotide 213, a silent substitution. At each of these positions, B4408 is identical to B46 B57 and may B15 alleles. As anticipated from its predicted iso-electric point (5.71), one-dimensional isoelectric focusing studies showed that B44BO focuses at the same position as B4402. The sequence and serological reactivity of this rare antigen allowed the identification of two likely epitopes shared by two different groups of HLA-B antigens.
我们利用HLA血清学方法在两个家族中检测到一种新的HLA - B44变异体——B44BO。该抗原与B44抗血清发生反应，与超过三分之一的B12（B44、B45）血清呈阴性反应，但与50%含有B62成分的抗血清发生反应，尤其是那些含有抗B57的抗血清。该变异体B4408与常见的B4402在外显子2中有4个核苷酸替换：193、206和209位，这些替换导致α1结构域中41、45和46位发生变化（B4402中的TKE和B44BO中的AMA）；以及213位的沉默替换。在这些位置上，B4408与B46、B57以及可能的B15等位基因相同。正如根据其预测的等电点（5.71）所预期的那样，一维等电聚焦研究表明B44BO与B4402聚焦在相同位置。这种罕见抗原的序列和血清学反应性使得能够鉴定出两组不同的HLA - B抗原共有的两个可能的表位。
