Vilches C, Bunce M, de Pablo R, Murray A K, McIntyre C A, Kreisler M
Servicio de Inmunología, Clínica Puerta de Hierro, Madrid, Spain.
Tissue Antigens. 1997 Jul;50(1):38-41. doi: 10.1111/j.1399-0039.1997.tb02831.x.
Two previously reported PCR-SSP variants of the HLA-B locus, B51GAC and B45v, were investigated by RT-PCR cloning and nucleotide sequence analysis of their complete coding regions. They have been shown to correspond to the new alleles B5108 and B5002, both of which differ from the common B5101 and B5001 subtypes, respectively, by amino acid replacements at their alpha-2 domain alpha-helices. The primary structure of B5002, intermediate between those of B4501 and B5001, raises further concern about the current classification of B45 as a B12 rather than as a B*50 subtype.
通过对HLA - B位点的两个先前报道的PCR - SSP变体B51GAC和B45v的完整编码区进行逆转录聚合酶链反应（RT - PCR）克隆和核苷酸序列分析，对它们展开了研究。结果表明它们分别对应新等位基因B5108和B5002，这两个新等位基因在其α - 2结构域α - 螺旋处的氨基酸替换，使其分别与常见的B5101和B5001亚型有所不同。B5002的一级结构处于B4501和B5001之间，这进一步引发了对于当前将B45归类为B12而非B*50亚型的质疑。
