Sobek-Klocke I, Disqué-Kochem C, Ronsiek M, Klocke R, Jockusch H, Breuning A, Ponstingl H, Rojas K, Overhauser J, Eichenlaub-Ritter U
Faculty of Biology, Genetechnology/Microbiology Unit, University of Bielefeld, Germany.
Genomics. 1997 Jul 15;43(2):156-64. doi: 10.1006/geno.1997.4784.
A clone from a lambda gt11 cDNA expression library of HeLa cells was isolated, sequenced, and shown to encode a new human zinc finger protein. The cDNA of the gene termed ZFP161 has an open reading frame of 1347 bp. The predicted protein comprises 449 amino acid residues and contains five zinc finger motifs of the Krüppel type near the C-terminus and a BTB/POZ domain in the N-terminal region. The protein is 98% homologous to a murine zinc finger protein, ZF5 (M. Numoto et al., 1993, Nucleic Acids Res. 21: 3767-3775), which is a putative transcriptional repressor of c-myc and exhibits growth-suppressive activity in mouse cell lines. Through the use of a panel of somatic cell hybrids for chromosomal assignment and DNAs of somatic cell hybrids containing a deleted chromosome 18 for fine mapping, the human gene ZFP161 was localized to 18p11.21-pter. Therefore, ZFP161 is a candidate gene by position for the holoprosencephaly type 4 gene, HPE4, which is involved in congenital malformations. With DNAs from an interspecific backcross, two homologous mouse genes, Zfp161 and Zfp161-rs1, were mapped to chromosome 17 and the X chromosome, respectively. Mapping of Zfp161 confirms and extends a region of homology between distal mouse chromosome 17 and human 18p.
从HeLa细胞的λgt11 cDNA表达文库中分离出一个克隆，进行测序后发现其编码一种新的人类锌指蛋白。名为ZFP161的该基因的cDNA具有1347 bp的开放阅读框。预测的蛋白质包含449个氨基酸残基，在C末端附近含有五个Krüppel型锌指基序，在N末端区域含有一个BTB/POZ结构域。该蛋白质与小鼠锌指蛋白ZF5（M. Numoto等人，1993年，《核酸研究》21: 3767 - 3775）有98%的同源性，ZF5是c-myc的假定转录抑制因子，在小鼠细胞系中表现出生长抑制活性。通过使用一组体细胞杂种进行染色体定位以及利用含有缺失18号染色体的体细胞杂种的DNA进行精细定位，人类基因ZFP161被定位到18p11.21 - pter。因此，ZFP161是4型前脑无裂畸形基因HPE4的一个位置候选基因，HPE4与先天性畸形有关。利用种间回交的DNA，两个同源的小鼠基因Zfp161和Zfp161 - rs1分别被定位到17号染色体和X染色体上。Zfp161的定位证实并扩展了小鼠17号染色体远端与人18p之间的同源区域。
