Cherdyntseva N V, Litviakov N V, Smol'ianinov E S, Beliavskaia V A, Masycheva V I
Vopr Onkol. 1997;43(3):313-6.
A new preparation "subalin" developed on the basis of a recombinant saprophyte strain of Bacillus subtilis 2135/105 producing human interferon alpha-2 has been tested for its ability of increasing the effectiveness of antitumor therapy. The study used Lewis lung carcinoma bearing mice C57B1/6 treated with injections of 60 mg/kg body cyclophosphamide, intraperitoneally, on days 3 and 7 of tumor growth. Subalin was administered per as on days 1-14 after tumor transplantation. Combined treatment with cyclophosphamide and subalin resulted in a significantly higher inhibition of primary tumor growth and metastatic spreading as compared with control receiving cyclophosphamide alone. Also, there were fewer animals with metastasis; the average number of these lesions per animal and the total affected area were less. Subalin proved to exert a moderate antitumor and antimetastatic effect. The cytostatic activity of peritoneal macrophages was higher in mice treated with subalin. It is suggested that the chemo-sensitizing and antitumor effects of subalin are due to its ability to induce endogenous interferon production.
一种基于产生人α-2干扰素的枯草芽孢杆菌2135/105重组腐生菌株研制的新制剂“苏巴林”，已对其增强抗肿瘤治疗效果的能力进行了测试。该研究使用了C57B1/6荷Lewis肺癌小鼠，在肿瘤生长的第3天和第7天腹腔注射60mg/kg体重的环磷酰胺进行治疗。在肿瘤移植后的第1至14天经口给予苏巴林。与单独接受环磷酰胺的对照组相比，环磷酰胺与苏巴林联合治疗对原发性肿瘤生长和转移扩散的抑制作用明显更强。此外，发生转移的动物数量更少；每只动物这些病变的平均数量和总受累面积更小。苏巴林被证明具有适度的抗肿瘤和抗转移作用。用苏巴林治疗的小鼠腹腔巨噬细胞的细胞抑制活性更高。有人认为，苏巴林的化学增敏和抗肿瘤作用归因于其诱导内源性干扰素产生的能力。
