Wehrly K, Chesebro B
Laboratory of Persistent, Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, NIH, Hamilton, Montana 59840-2932, USA.
Methods. 1997 Aug;12(4):288-93. doi: 10.1006/meth.1997.0481.
Antigen capture enzyme-linked immunosorbent assay (ELISA) for quantitation of the p24 gag protein of human immunodeficiency virus type-1 (HIV-1) is currently the most common method used to demonstrate virus replication both in vivo and in vitro. The present paper describes an ELISA employing readily available inexpensive reagents and gives detailed suggestions for optimizing the variables for specific purposes. The assay is as sensitive as commercial kits (25 pg/ml) and has a linear dose response over a wide range of p24 concentrations (25-1000 pg/ml). For these reasons, as well as its low cost, this assay has proven useful in a variety of research applications. This assay also has been found to be effective in detecting the gag protein of human immunodeficiency virus type-2 and simian immunodeficiency virus.
用于定量人类免疫缺陷病毒1型（HIV-1）p24 gag蛋白的抗原捕获酶联免疫吸附测定（ELISA）是目前用于证明体内和体外病毒复制的最常用方法。本文描述了一种使用易于获得的廉价试剂的ELISA，并针对特定目的优化变量给出了详细建议。该测定与商业试剂盒一样灵敏（25 pg/ml），并且在广泛的p24浓度范围（25-1000 pg/ml）内具有线性剂量反应。由于这些原因以及其低成本，该测定已被证明在各种研究应用中有用。该测定还被发现可有效检测人类免疫缺陷病毒2型和猿猴免疫缺陷病毒的gag蛋白。
