Wetzer B, Pum D, Sleytr U B
Zentrum für Ultrastrukturforschung, Universität für Bodenkultur, Vienna, Austria.
J Struct Biol. 1997 Jul;119(2):123-8. doi: 10.1006/jsbi.1997.3867.
This work describes composite structures composed of lipid bilayer or tetraetherlipid monolayer films attached to solid supports with associated crystalline bacterial cell surface layers (S-layers). The bilayer system was established by making use of the strong chemisorption of a first monolayer of thiolipids (1-octadecanethiol or 1,2-dimyristoyl-sn-glycero-3-phosphothioethanol) on gold and attaching a second monolayer of 1,2-dipalmitoyl-sn-3-phosphatidylethanolamine by the Langmuir Schaefer technique. The tetraetherlipid monolayer was composed of Glycerol-dialkyl-nonitol tetraetherlipid (GDNT). The monolayer of GDNT exhibits the thickness of a bilayer with hydrophilic headgroups on both sides and a hydrophobic inner part. Isolated S-layer protein from Bacillus sphaericus (CCM2177, which was injected into the subphase of an LB-trough, recrystallized into a coherent monolayer at the solid supported phospholipid bilayer and at the tetraehtherlipid monolayer. The composite lipid/S-layer structures were stable enough to allow lifting from the air-water interface, rinsing in water, and transfer into a scanning force microscope.
这项工作描述了由脂质双层或四醚脂质单层膜组成的复合结构，这些膜附着在带有相关结晶细菌细胞表面层（S层）的固体支持物上。通过利用硫脂质（1-十八烷硫醇或1,2-二肉豆蔻酰-sn-甘油-3-磷硫乙醇）的第一单层在金上的强化学吸附，并通过朗缪尔-谢弗技术附着1,2-二棕榈酰-sn-3-磷脂酰乙醇胺的第二单层，建立了双层系统。四醚脂质单层由甘油二烷基壬糖醇四醚脂质（GDNT）组成。GDNT单层表现出双层的厚度，两侧具有亲水头基，内部为疏水部分。从球形芽孢杆菌（CCM2177）分离出的S层蛋白被注入LB槽的亚相中，在固体支持的磷脂双层和四醚脂质单层上重结晶为连贯的单层。复合脂质/S层结构足够稳定，能够从气-水界面提起，在水中冲洗，并转移到扫描力显微镜中。
