Dillon S M, Hart D N, Abernethy N, Watson J D, Baird M A
Department of Pathology, Dunedin School of Medicine, University of Otago, New Zealand.
Scand J Immunol. 1997 Jul;46(1):1-9. doi: 10.1046/j.1365-3083.1997.d01-88.x.
Antigen presenting cells (APC) similar to immature dendritic cells can be generated in vitro from bone marrow precursors. The authors have compared the yield, the phenotype and the function of murine bone marrow cells cultured for 7 or 11 days in either granulocyte macrophage colony stimulating factor alone (GM BMAPC) or in combination with interleukin-4 (GM/IL-4 BMAPC). The results showed that GM/IL-4 BMAPC expressed the highest levels of MHC Class 2 molecules, CD86/B7-2 and CD80/B7-1 co-stimulatory molecules and the lowest levels of F4/80 macrophage marker. However, when these APC were pulsed with BCG culture filtrate antigen or PPD they were not correspondingly more effective at stimulating activated T lymphocytes in vitro or priming naive T lymphocytes in vivo. Also, in contrast to GM BMAPC, high backgrounds recorded following injections of GM/IL-4 BMAPC without antigen were not consistently reduced by lowering the dose and irradiating the cells prior to administration. The authors conclude that the degree of maturity of BMAPC varies with culture conditions and that this may be an important consideration where BMAPC are to be used in vivo in immunotherapeutic regimens.
类似于未成熟树突状细胞的抗原呈递细胞（APC）可在体外由骨髓前体细胞生成。作者比较了单独在粒细胞巨噬细胞集落刺激因子（GM BMAPC）中或与白细胞介素-4联合培养（GM/IL-4 BMAPC）7天或11天的小鼠骨髓细胞的产量、表型和功能。结果显示，GM/IL-4 BMAPC表达最高水平的MHC II类分子、CD86/B7-2和CD80/B7-1共刺激分子，以及最低水平的F4/80巨噬细胞标志物。然而，当用卡介苗培养滤液抗原或PPD刺激这些APC时，它们在体外刺激活化T淋巴细胞或在体内启动幼稚T淋巴细胞方面并没有相应地更有效。此外，与GM BMAPC相反，在注射无抗原的GM/IL-4 BMAPC后记录到的高背景，在给药前降低剂量并照射细胞后并没有持续降低。作者得出结论，BMAPC的成熟程度随培养条件而变化，这在免疫治疗方案中体内使用BMAPC时可能是一个重要的考虑因素。
