Greiner R, Konietzny U, Jany K D
Bundesforschungsanstalt für Ernährung, Karlsruhe, Germany.
Z Ernahrungswiss. 1997 Jun;36(2):155-60. doi: 10.1007/BF01611394.
To elucidate if there is any possibility to identify highly processed foods as produced through genetic engineering, beer, soya bean oil, processed tomato (ketch-up, paste, pizza tomatoes, peeled tomatoes, soup) and potato (french fries, crisps, mashed potatoes, flour, starch, fried potatoes) products as well as an enzyme preparation (Natuphos) were investigated by PCR. In pizza tomatoes, peeled tomatoes, french fries, fried potatoes, potato flour and potato crisps DNA suitable for PCR was found. Therefore, it is possible to identify these products as produced through genetic engineering. Such an identification is impossible in certain beers (pilsener, export, Nutfield lyte), soya bean oil, tomato soup, potato starch, mashed potatoes and Natuphos since PCR-analysis gave no indication of the presence of DNA in these products. As it was shown by adding Escherichia coli DNA the used method is, in principle, capable of detecting specifically small amounts of DNA in such products.
为了阐明是否有可能鉴定出通过基因工程生产的高度加工食品,对啤酒、大豆油、加工番茄(番茄酱、番茄糊、披萨用番茄、去皮番茄、番茄汤)和马铃薯(薯条、薯片、土豆泥、面粉、淀粉、炸土豆)产品以及一种酶制剂(耐特菲酶)进行了聚合酶链式反应(PCR)检测。在披萨用番茄、去皮番茄、薯条、炸土豆、马铃薯粉和薯片当中发现了适合进行PCR的DNA。因此,有可能鉴定出这些产品是通过基因工程生产的。对于某些啤酒(比尔森啤酒、出口啤酒、纽菲尔德淡啤)、大豆油、番茄汤、马铃薯淀粉、土豆泥和耐特菲酶而言,这种鉴定是不可能的,因为PCR分析没有显示这些产品中存在DNA。通过添加大肠杆菌DNA表明,所使用的方法原则上能够特异性检测此类产品中的少量DNA。