Satoh F, Takahashi K, Murakami O, Totsune K, Ohneda M, Mizuno Y, Sone M, Miura Y, Takase S, Hayashi Y, Sasano H, Mouri T
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendal, Japan.
J Endocrinol. 1997 Jul;154(1):27-34. doi: 10.1677/joe.0.1540027.
The expression of cerebellin and cerebellin mRNA was studied by radioimmunoassay and Northern blot analysis in the human brain, adrenal gland and the tumour tissues of adrenal tumour, ganglioneuroblastoma and neuroblastoma. Immunoreactive cerebellin was detected in every region of brain studied, with the highest concentrations found in the hemisphere of the cerebellum (424.2 +/- 12.6 pmol/g wet weight, n = 6, mean +/- S.E.M.) and the vermis of the cerebellum (256.8 +/- 30.5 pmol/g wet weight). Immunoreactive cerebellin was also detected in the pituitary (8.2 +/- 1.8 pmol/g wet weight), the spinal cord (3.3 +/- 0.3 pmol/g wet weight) and the normal parts of adrenal glands (2.98 +/- 0.37 pmol/g wet weight, n = 9) and some tumour tissues, such as phaeochromocytomas, cortisol-producing adrenocortical adenomas, ganglioneuroblastomas and neuroblastomas. Northern blot analysis showed that cerebellin mRNA was highly expressed in the hemisphere and vermis of the cerebellum. Cerebellin mRNA was also expressed in other regions of the brain and the tumour tissues of phaeochromocytoma, cortisol-producing adrenocortical adenoma, ganglioneuroblastoma and neuroblastoma. Immunocytochemistry of the normal adrenal gland showed that immunoreactive cerebellin was localized in the adrenal medulla. The present study has shown the expression of cerebellin and cerebellin mRNA, not only in the cerebellum but also in other regions of the brain and some tumours, such as cortisol-producing adrenocortical adenoma, phaeochromocytoma and neuroblastoma. These findings suggest possible pathophysiological roles of cerebellin peptides, not only in the cerebellum, but also in the extra-cerebellar tissues.
采用放射免疫分析法和Northern印迹分析法，对人脑中的小脑素及其mRNA、肾上腺以及肾上腺肿瘤、神经节神经母细胞瘤和神经母细胞瘤的肿瘤组织进行了研究。在所研究的脑的各个区域均检测到了免疫反应性小脑素，其中小脑半球（424.2±12.6 pmol/g湿重，n = 6，平均值±标准误）和小脑蚓部（256.8±30.5 pmol/g湿重）中的浓度最高。在垂体（8.2±1.8 pmol/g湿重）、脊髓（3.3±0.3 pmol/g湿重）、肾上腺正常部分（2.98±0.37 pmol/g湿重，n = 9）以及一些肿瘤组织，如嗜铬细胞瘤、产生皮质醇的肾上腺皮质腺瘤、神经节神经母细胞瘤和神经母细胞瘤中也检测到了免疫反应性小脑素。Northern印迹分析表明，小脑素mRNA在小脑半球和蚓部中高表达。小脑素mRNA也在脑的其他区域以及嗜铬细胞瘤、产生皮质醇的肾上腺皮质腺瘤、神经节神经母细胞瘤和神经母细胞瘤的肿瘤组织中表达。正常肾上腺的免疫细胞化学显示，免疫反应性小脑素定位于肾上腺髓质。本研究表明，小脑素及其mRNA不仅在小脑中表达，在脑的其他区域以及一些肿瘤，如产生皮质醇的肾上腺皮质腺瘤、嗜铬细胞瘤和神经母细胞瘤中也有表达。这些发现提示小脑素肽不仅在小脑中，而且在小脑外组织中可能具有病理生理作用。
