Byrd J C, Yan P, Sternberg L, Yunker C K, Scheiman J M, Bresalier R S
Gastrointestinal Cancer Research Laboratory, Henry Ford Health Sciences Center, Detroit, Michigan, USA.
Gastroenterology. 1997 Aug;113(2):455-64. doi: 10.1053/gast.1997.v113.pm9247464.
BACKGROUND & AIMS: Helicobacter pylori is found within the gastric mucous gel layer and in association with the epithelial surface. The aim of this study was to determine the effect of H. pylori infection on mucin gene expression (MUC6, MUC5, and MUC1) in the gastric epithelium.
Gastric biopsy specimens were examined by immunohistochemistry and in situ hybridization for mucin gene expression.
MUC6 was limited to mucous glands of H. pylori-negative patients, but 72% of H. pylori-positive patients also expressed MUC6 in surface mucous cells. In contrast, MUC5 mucin was found in significantly fewer surface mucous cells of H. pylori-positive specimens. Carbohydrates recognized by Lewis (Le) X and paradoxical concanavalin A (con A) staining were aberrantly expressed in the surface mucous cells of 16 of 27 and 17 of 23 H. pylori-positive tissues, respectively. Surface Le(b) was present in 26 of 27 H. pylori-positive and 19 of 30 H. pylori-negative tissues. Eradication of H. pylori resulted in reversal of surface MUC5 and MUC6 expression toward normal patterns. Purified gastric mucins of H. pylori-positive patients bound more anti-MUC6 and anti-Le(b) than mucins from H. pylori-negative patients.
The type of mucin that is normally limited to mucous glands is aberrantly expressed in surface mucous cells of H. pylori-positive patients, whereas less of the surface epithelium expresses normal surface-type gastric mucin.
幽门螺杆菌存在于胃黏液凝胶层并与上皮表面相关联。本研究的目的是确定幽门螺杆菌感染对胃上皮中黏蛋白基因表达（MUC6、MUC5和MUC1）的影响。
通过免疫组织化学和原位杂交检查胃活检标本中的黏蛋白基因表达。
MUC6仅限于幽门螺杆菌阴性患者的黏液腺，但72%的幽门螺杆菌阳性患者的表面黏液细胞中也表达MUC6。相比之下，在幽门螺杆菌阳性标本的表面黏液细胞中发现MUC5黏蛋白明显较少。分别在27个幽门螺杆菌阳性组织中的16个和23个中的17个的表面黏液细胞中，Lewis（Le）X和反常伴刀豆球蛋白A（伴刀豆球蛋白A）染色识别的碳水化合物异常表达。27个幽门螺杆菌阳性组织中的26个和30个幽门螺杆菌阴性组织中的19个存在表面Le(b)。根除幽门螺杆菌导致表面MUC5和MUC6表达向正常模式逆转。幽门螺杆菌阳性患者的纯化胃黏蛋白比幽门螺杆菌阴性患者的黏蛋白结合更多的抗MUC6和抗Le(b)。
通常仅限于黏液腺的黏蛋白类型在幽门螺杆菌阳性患者的表面黏液细胞中异常表达，而较少的表面上皮表达正常的表面型胃黏蛋白。
