Morgenstern S, Koren R, Moss S F, Fraser G, Okon E, Niv Y
Department of Pathology, Rabin Medical Center, Tel Aviv University, Israel.
Eur J Gastroenterol Hepatol. 2001 Jan;13(1):19-23. doi: 10.1097/00042737-200101000-00004.
BACKGROUND/OBJECTIVE: Helicobacter pylori colonizes the gastric mucous gel layer, the surface epithelium and glands. It has been shown that H. pylori infection causes aberrant expression of gastric mucins MUC 5 and MUC 6. This study aimed to determine the distribution of MUC 5 and MUC 6 in the gastric antrum of dyspeptic patients, and to investigate changes in this pattern in the presence of H. pylori and after successful eradication.
Gastric antrum biopsy specimens were examined by immunohistochemistry for mucin gene (MUC 5 and MUC 6) expression. Polyclonal antibodies were used to detect amino acid tandem repeats of each protein. A scoring system (0-3) was used to assess staining intensity at three sites: foveola, mucous neck cells and glands. H. pylori status was determined by histology and rapid urease test, and considered positive or negative when both tests were positive or negative, respectively. The study included 49 patients positive for H. pylori, in 36 of whom successful eradication was performed, and 11 H. pylori-negative patients.
There was a gradient of MUC 5 expression, higher to lower, from the surface to the glands, which was more pronounced before eradication. Increased MUC 5 synthesis in the mucous neck cells and in the glands was found after H. pylori eradication (P = 0.016). MUC 6 was synthesized in the glands more than in the mucous neck cells or foveola. MUC 6 was also secreted into the lumen and probably comprised the superficial part of the unstirred mucous layer.
The change in MUC 5 synthesis may reflect H. pylori colonization.
背景/目的：幽门螺杆菌定殖于胃黏液凝胶层、表面上皮和腺体。已有研究表明，幽门螺杆菌感染会导致胃黏蛋白MUC 5和MUC 6表达异常。本研究旨在确定消化不良患者胃窦中MUC 5和MUC 6的分布，并研究在幽门螺杆菌存在时以及成功根除后这种模式的变化。
通过免疫组织化学检查胃窦活检标本中黏蛋白基因（MUC 5和MUC 6）的表达。使用多克隆抗体检测每种蛋白质的氨基酸串联重复序列。采用评分系统（0 - 3）评估三个部位（小凹、黏液颈细胞和腺体）的染色强度。通过组织学和快速尿素酶试验确定幽门螺杆菌状态，当两项试验均为阳性或均为阴性时，分别视为阳性或阴性。该研究包括49例幽门螺杆菌阳性患者，其中36例成功根除幽门螺杆菌，以及11例幽门螺杆菌阴性患者。
MUC 5表达存在从表面到腺体由高到低的梯度，在根除前更为明显。根除幽门螺杆菌后，黏液颈细胞和腺体中MUC 5合成增加（P = 0.016）。MUC 6在腺体中的合成多于黏液颈细胞或小凹。MUC 6也分泌到管腔中，可能构成未搅动黏液层的表层部分。
MUC 5合成的变化可能反映幽门螺杆菌的定殖情况。
