Ota H, Nakayama J, Momose M, Hayama M, Akamatsu T, Katsuyama T, Graham D Y, Genta R M
Department of Medicine, Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Tex, USA.
Virchows Arch. 1998 Nov;433(5):419-26. doi: 10.1007/s004280050269.
The protective ability of gastric mucins may depend largely on their oligosaccharide chains. We evaluated the effects of H. pylori infection on the glycosylation of gastric mucins. Gastric biopsy specimens from 20 H. pylori-infected patients before and after cure of the H. pylori infection and 8 normal uninfected volunteers were examined by immunostaining for simple mucin-type glycoproteins and blood-group-related antigens bearing type 1 chain backbone. The immunoreactivity in different gastric compartments was evaluated. Simple mucin-type glycoproteins and blood-group-related antigens were expressed in surface mucous cells. Simple mucin-type glycoproteins showed antrum-predominant expression in normal volunteers and were found in significantly fewer surface mucous cells in infected patients than in normal volunteers; their expression was restored after eradication of H. pylori. Sialyl Lewis(a) and Lewis(b) were expressed in fewer surface mucous cells after than before eradication. The patterns of glycosylation of gastric mucins vary in different gastric compartments and are reversibly altered by H. pylori infection. These alterations may affect the protective functions of gastric mucins.
胃黏液素的保护能力可能在很大程度上取决于其寡糖链。我们评估了幽门螺杆菌感染对胃黏液素糖基化的影响。通过对简单黏蛋白型糖蛋白和带有1型链骨架的血型相关抗原进行免疫染色，检查了20例幽门螺杆菌感染患者在幽门螺杆菌感染治愈前后的胃活检标本以及8名正常未感染志愿者的标本。评估了不同胃区域的免疫反应性。简单黏蛋白型糖蛋白和血型相关抗原在表面黏液细胞中表达。简单黏蛋白型糖蛋白在正常志愿者中表现为胃窦优势表达，在感染患者的表面黏液细胞中发现的数量明显少于正常志愿者；在根除幽门螺杆菌后其表达得以恢复。唾液酸化路易斯（a）和路易斯（b）在根除后比根除前在更少的表面黏液细胞中表达。胃黏液素的糖基化模式在不同胃区域有所不同，并因幽门螺杆菌感染而可逆性改变。这些改变可能会影响胃黏液素的保护功能。
