McColl K A, Gogolewski R P, Chin J C
Elizabeth McArthur Agricultural Institute, NSW Department of Agriculture, Menangle.
Aust Vet J. 1997 Jun;75(6):421-3. doi: 10.1111/j.1751-0813.1997.tb14346.x.
To compare haematological values and lymphocyte phenotypes in the peripheral blood of fleece rot-resistant and -susceptible sheep.
Experiments were conducted on 2- and 3-year-old Merino rams, flock 1 (17 rams) and flock 2 (32 rams), respectively. Within each flock, individual rams were classified as fleece rot-resistant or -susceptible, based on established criteria. Total and differential white cell counts, and indirect fluorescent antibody tests specific for B cells and T cells were performed on all sheep. The concentration of various subsets of circulating lymphocytes was then determined in each sheep.
There were no significant differences between fleece rot-resistant and -susceptible sheep from either flock in the mean total or differential white cell counts. However, fleece rot-resistant rams in flock 1 did have a significantly higher concentration of circulating SBU-T1+ cells than fleece rot-susceptible rams from the same flock. No such difference was noted in the rams from flock 2. While all rams in flock 1 were free of clinical fleece rot, 24 rams in flock 2 (comprising all 17 fleece rot-susceptible and 7 of 15 fleece rot-resistant animals) had clinical signs of the disease. Fleece rot-free rams in this flock (irrespective of their classification as fleece rot-resistant or -susceptible) had significantly higher concentrations of circulating SBU-T1+ cells compared with fleece rot-affected animals. They also had significantly higher concentrations of circulating B cells, and total lymphocytes.
An examination of peripheral blood lymphocyte subsets in fleece rot-resistant and -susceptible sheep revealed a possible association between resistance to fleece rot and the concentration of circulating SBU-T1+ cells.
比较抗羊毛腐烂和易患羊毛腐烂绵羊外周血中的血液学指标及淋巴细胞表型。
分别对2岁和3岁的美利奴公羊进行实验，1群（17只公羊）和2群（32只公羊）。在每个羊群中，根据既定标准将个体公羊分为抗羊毛腐烂或易患羊毛腐烂。对所有绵羊进行白细胞总数和分类计数，以及针对B细胞和T细胞的间接荧光抗体检测。然后测定每只绵羊循环淋巴细胞各亚群的浓度。
两群中抗羊毛腐烂和易患羊毛腐烂的绵羊在白细胞总数或分类计数的平均值上均无显著差异。然而，1群中抗羊毛腐烂的公羊循环SBU-T1+细胞的浓度显著高于同一羊群中易患羊毛腐烂的公羊。2群的公羊中未观察到这种差异。虽然1群中的所有公羊均无临床羊毛腐烂症状，但2群中的24只公羊（包括所有17只易患羊毛腐烂的羊和15只抗羊毛腐烂羊中的7只）有该病的临床症状。与受羊毛腐烂影响的动物相比，该群中无羊毛腐烂症状的公羊（无论其分类为抗羊毛腐烂或易患羊毛腐烂）循环SBU-T1+细胞的浓度显著更高。它们循环B细胞和淋巴细胞总数的浓度也显著更高。
对抗羊毛腐烂和易患羊毛腐烂绵羊的外周血淋巴细胞亚群进行检查发现，抗羊毛腐烂能力与循环SBU-T1+细胞的浓度之间可能存在关联。
