Otsuka Y, Takano T S, Yamazaki T
Division of Medical Zoology, Oita Medical University, Japan.
Genes Genet Syst. 1997 Feb;72(1):19-24. doi: 10.1266/ggs.72.19.
Genetic variation in total mRNA level of the six hsp genes (hsp22, hsp23, hsp26, hsp27, hsp70 and hsp82) in the presence of heat shock was investigated by using seventy-four second- and seventy third-chromosome lines of Drosophila melanogaster which have the same genetic background derived from a highly inbred stock. There was significant variation in all the six hsp genes for both the second- and third-chromosome lines except for the hsp22 gene of the third-chromosome lines. Although all the structural genes of the heat shock proteins are localized on the third chromosome, the estimates of genetic variance for the second-chromosome lines were larger than those for the third-chromosome lines. Highly significant correlations between total mRNA level of the different hsp genes in all the combinations of the six hsp genes using the second-chromosome lines were found, but some of correlations for the third-chromosome lines were not significant. These results suggest that some second chromosome variants have similar effects on the expression of the different genes.
利用74个第二染色体品系和70个第三染色体品系的黑腹果蝇进行研究，这些品系具有相同的遗传背景，均源自一个高度近交的种群，以此来探究在热休克条件下六个热休克蛋白基因（hsp22、hsp23、hsp26、hsp27、hsp70和hsp82）的总mRNA水平的遗传变异。除了第三染色体品系的hsp22基因外，第二染色体品系和第三染色体品系的所有六个热休克蛋白基因均存在显著变异。尽管热休克蛋白的所有结构基因都定位在第三染色体上，但第二染色体品系的遗传方差估计值大于第三染色体品系。使用第二染色体品系发现，六个热休克蛋白基因的所有组合中不同热休克蛋白基因的总mRNA水平之间存在高度显著的相关性，但第三染色体品系的一些相关性并不显著。这些结果表明，一些第二染色体变异对不同基因的表达具有相似的影响。
