Vitek M P, Berger E M
J Mol Biol. 1984 Sep 15;178(2):173-89. doi: 10.1016/0022-2836(84)90138-4.
Transcription of the four small heat-shock protein genes of Drosophila melanogaster can be induced in cultured cells by high-temperature shock, or by physiological doses of the moulting hormone, ecdysterone. We have characterized and compared the two induction events, focusing on hsp22 and hsp23, in terms of rates of heat-shock protein synthesis, transcription rate, messenger RNA abundance and mRNA half-life. The results indicate that relative to hsp22, the rate of hsp23 synthesis is significantly greater during recovery from heat shock and during ecdysterone induction. This difference is not due to differences in transcription rate, but rather reflects differences in mRNA stability and translational efficiency. One intriguing finding is that hsp message stability is temperature-dependent; hsp transcripts are two to three times more stable at 35 degrees C than at 25 degrees C. The possible mechanism and significance of this phenomenon are discussed.
黑腹果蝇的四个小热休克蛋白基因的转录可通过高温休克或生理剂量的蜕皮激素（蜕皮甾酮）在培养细胞中诱导产生。我们对这两种诱导事件进行了表征和比较，重点关注hsp22和hsp23，涉及热休克蛋白合成速率、转录速率、信使RNA丰度和mRNA半衰期。结果表明，相对于hsp22，在热休克恢复期间和蜕皮甾酮诱导期间，hsp23的合成速率显著更高。这种差异不是由于转录速率的不同，而是反映了mRNA稳定性和翻译效率的差异。一个有趣的发现是，hsp信息稳定性取决于温度；hsp转录本在35摄氏度时的稳定性是25摄氏度时的两到三倍。讨论了这种现象可能的机制和意义。
