Jayaraman A, Cheng E T, Earthman J C, Wood T K
Department of Chemical and Biochemical Engineering & Materials Science, University of California, Irvine 92697-2575, USA.
J Ind Microbiol Biotechnol. 1997 Jun;18(6):396-401. doi: 10.1038/sj.jim.2900396.
To investigate if corrosion inhibition by aerobic biofilms is a general phenomenon, carbon steel (SAE 1018) coupons were exposed to a complex liquid medium (Luria-Bertani) and seawater-mimicking medium (VNSS) containing fifteen different pure-culture bacterial suspensions representing seven genera. Compared to sterile controls, the mass loss in the presence of these bacteria (which are capable of developing a biofilm to various degrees) decreased by 2- to 15-fold. The extent of corrosion inhibition in LB medium depended on the nature of the biofilm: an increased proportion of live cells, observed with confocal scanning laser microscopy (CSLM) and image analysis, decreased corrosion. Corrosion inhibition in LB medium was greatest with Pseudomonas putida (good biofilm formation), while metal coupons exposed to Streptomyces lividans in LB medium (poor biofilm formation) corroded in a manner similar to the sterile controls. Pseudomonas mendocina KR1 reduced corrosion the most in VNSS. It appears that only a small layer of active, respiring cells is required to inhibit corrosion, and the corrosion inhibition observed is due to the attached biofilm.
为了研究需氧生物膜的缓蚀作用是否是一种普遍现象，将碳钢（SAE 1018）试片暴露于含有代表七个属的15种不同纯培养细菌悬液的复合液体培养基（Luria-Bertani）和模拟海水培养基（VNSS）中。与无菌对照相比，在这些能够不同程度形成生物膜的细菌存在下，质量损失降低了2至15倍。LB培养基中的缓蚀程度取决于生物膜的性质：通过共聚焦扫描激光显微镜（CSLM）和图像分析观察到的活细胞比例增加，腐蚀减少。恶臭假单胞菌（生物膜形成良好）在LB培养基中的缓蚀作用最强，而暴露于LB培养基中的变铅青链霉菌（生物膜形成较差）的金属试片的腐蚀方式与无菌对照相似。门多萨假单胞菌KR1在VNSS中对腐蚀的降低作用最大。似乎仅需一小层活跃的、进行呼吸作用的细胞就能抑制腐蚀，观察到的缓蚀作用归因于附着的生物膜。
