Gillings M, Holley M
Key Centre for Biodiversity and Bioresources, School of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Lett Appl Microbiol. 1997 Jul;25(1):17-21. doi: 10.1046/j.1472-765x.1997.00162.x.
We examined the use of enterobacterial repetitive intergenic consensus (ERIC) sequences in PCR on the DNAs of various bacteria, bacteriophage, invertebrates, fungi, plants and vertebrates and have shown that complex ERIC-PCR patterns can be readily produced from all of these target organisms. A range of annealing temperatures was tested, from 52 degrees C (the commonly used annealing temperature) to 66 degrees C (the approximate Tm of ERIC primers). At the higher temperatures, most bands failed to amplify, the exception being a subset of bands from enterobacterial targets. It was concluded that ERIC-PCR does not necessarily direct amplification from genuine ERIC sequences.
我们检测了肠杆菌基因间重复一致序列（ERIC）在各种细菌、噬菌体、无脊椎动物、真菌、植物和脊椎动物DNA的PCR中的应用，结果表明，所有这些目标生物均能轻易产生复杂的ERIC-PCR图谱。我们测试了一系列退火温度，从52℃（常用的退火温度）到66℃（ERIC引物的近似熔解温度）。在较高温度下，大多数条带未能扩增，例外的是来自肠道细菌目标的一部分条带。由此得出结论，ERIC-PCR不一定直接从真正的ERIC序列进行扩增。
