Karimi Kaivan, Arzanlou Mahdi, Pertot Ilaria
Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
Department of Sustainable Agro-Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy.
Front Microbiol. 2019 Feb 12;10:129. doi: 10.3389/fmicb.2019.00129. eCollection 2019.
Strawberry anthracnose caused by spp. is considered one of the most serious and destructive disease of strawberry worldwide. Weeds, as possible hosts of the pathogen, could have a role as potential inoculum reservoir. To prove this hypothesis, symptomless weeds were collected in strawberry fields showing anthracnose symptoms in Iran. Ten isolates with -like colonies were recovered from symptomless L., L., L., L., and L. plants. The isolates were identified as , based on a combination of morphological and sequence data of TUB and GADPH genes. This identification was further validated using Rep-PCR fingerprinting analysis, which produces species-specific DNA fingerprints and unveils inter and intra variation of the species examined in this study. Moreover, rep-PCR marker was used to reveal accurate taxonomic position of spp. causing strawberry anthracnose belonging to the complex, including , and . The isolates originating from symptomless weeds confirmed their pathogenicity on detached strawberry, proving that weeds in strawberry field may have a role as reservoir of inoculum. However, further studies are necessary to quantify their actual contribution to anthracnose epidemics in strawberry fields.
由[具体病原菌名称]引起的草莓炭疽病被认为是全球草莓最严重且具毁灭性的病害之一。杂草作为病原菌的可能寄主，可能充当潜在的接种体库。为证明这一假设，在伊朗有炭疽病症状的草莓田采集了无症状杂草。从无症状的[多种杂草学名]植株上分离出了10个具有[菌落特征]样菌落的分离株。基于TUB和GADPH基因的形态学和序列数据组合，这些分离株被鉴定为[具体病原菌名称]。使用Rep-PCR指纹分析进一步验证了该鉴定结果，Rep-PCR可产生物种特异性DNA指纹，并揭示本研究中所检测物种的种间和种内变异。此外，rep-PCR标记用于揭示引起草莓炭疽病的[具体病原菌名称]种在[相关复合体名称]复合体中的准确分类地位，该复合体包括[相关种的名称]。源自无症状杂草的[具体病原菌名称]分离株在离体草莓上证实了其致病性，证明草莓田中的杂草可能充当接种体库。然而，需要进一步研究来量化它们对草莓田炭疽病流行的实际贡献。
