Benschop H P, van der Schans G P, Noort D, Fidder A, Mars-Groenendijk R H, de Jong L P
TNO Prins Maurits Laboratory, AA Rijswijk, The Netherlands.
J Anal Toxicol. 1997 Jul-Aug;21(4):249-51. doi: 10.1093/jat/21.4.249.
The exposure of two Iranian victims of the Iran-Iraq conflict (1980-1988) to sulfur mustard was established by immunochemical and mass spectrometric analysis of blood samples taken 22 and 26 days after alleged exposure. One victim suffered from skin injuries compatible with sulfur mustard intoxication but did not have lung injuries; the symptoms of the other victim were only vaguely compatible with sulfur mustard intoxication. Both patients recovered. Immunochemical analysis was based on detection of the N7-guanine adduct of the agent in DNA from lymphocytes and granulocytes, whereas the N-terminal valine adduct in globin was determined by gas chromatography-mass spectrometry after a modified Edman degradation. The valine adduct levels correspond with those found in human blood after in vitro treatment with 0.9 microM sulfur mustard.
通过对据称接触后22天和26天采集的血样进行免疫化学和质谱分析，确定了两名为伊朗-伊拉克冲突（1980 - 1988年）受害者的伊朗人接触了芥子气。一名受害者有与芥子气中毒相符的皮肤损伤，但没有肺部损伤；另一名受害者的症状仅与芥子气中毒大致相符。两名患者均康复。免疫化学分析基于检测淋巴细胞和粒细胞DNA中该制剂的N7 - 鸟嘌呤加合物，而通过改良的埃德曼降解后，用气相色谱 - 质谱法测定球蛋白中的N - 末端缬氨酸加合物。缬氨酸加合物水平与用0.9微摩尔芥子气进行体外处理后人血中发现的水平相符。
